EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA IN THE RAT VENTRAL PROSTATE DURING CASTRATION-INDUCED PROGRAMMED CELL-DEATH
EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA IN THE RAT VENTRAL PROSTATE DURING CASTRATION-INDUCED PROGRAMMED CELL-DEATH
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DOI：
10.1210/mend-3-10-1515
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发表时间：
1989-10-01
影响因子：
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通讯作者：
ISAACS, JT
中科院分区：
文献类型：
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作者：
KYPRIANOU, N;ISAACS, JT
去势诱导的雄激素剥夺导致大鼠腹侧前列腺雄激素依赖性前列腺上皮细胞程序性死亡的激活。为了确定这种程序性细胞死亡的潜在介质，转化生长因子- β的表达。（tgf . β）在去势诱导雄激素戒断大鼠腹侧前列腺中的表达。tgf - β的稳态水平。通过Northern blot分析测定mRNA，并将mRNA水平与前列腺前腹主要雄激素依赖性分泌蛋白前列腺素C3的mRNA水平和TRPM-2的mRNA水平进行比较，TRPM-2是去势诱导前列腺细胞死亡时特异性表达的基因。在阉割后的第一天内，tgf - β的水平急剧上升。前列腺腹侧mRNA的表达(.apprx。10倍)和去势后4天tgf . β。mRNA最大表达量（.apprx）。增加40倍)，此时雄激素依赖性C3分泌蛋白mRNA转录物已经减少到无法检测的水平。阉割4天的大鼠给予雄激素后，tgf . β显著降低。mRNA的表达水平与其在完整对照动物中的组成表达水平相当，表明tgf . β。大鼠腹侧前列腺受负雄激素调节。编码TRPM-2的转录本水平在去势后第一天增加了10倍，到去势后第4天急剧增加（约50倍），这与雄激素依赖性前列腺上皮细胞的最大细胞死亡率密切相关。这些结果表明tgf . β的表达在时间上存在相关性。去势诱导雄激素戒断后大鼠腹侧前列腺细胞程序性死亡的转录本和激活。
Castration-induced androgen deprivation leads to the activation of the programmed death of the androgen-dependent prostatic epithelial cells in the rat ventral prostate. In order to identify potential mediators of this programmed cell death, the expression of transforming growth factor-.beta. (TGF.beta.) in the rat ventral prostate was studied, after castration induced-androgen withdrawal. Steady state levels of TGF.beta. mRNA were determined by Northern blot analysis and compared with mRNA levels for prostatein C3, the major androgen-dependent secretory protein of ventral prostate and also with mRNA levels for TRPM-2, a gene that is specifically expressed during castration induced prostatic cell death. Within the first day after castration there was a dramatic increase in the levels of TGF.beta. mRNA in the ventral prostate (.apprx. 10-fold) and by 4 days after castration TGF.beta. mRNA was maximally expressed (.apprx.40-fold increase), by which time the androgen-dependent C3 secretory protein mRNA transcripts have diminished to undetectable levels. Androgen administration to 4-day castrated rats led to a marked decrease in TGF.beta. mRNA to a level comparable to its constitutive expression obtained in the intact control animals, indicating that expression of TGF.beta. in the rat ventral prostate is under negative androgenic regulation. The transcript levels encoding TRPM-2 initially increased 10-fold within the first day after castration and by day 4 post castration there was a dramatic increase (.apprx.50-fold) which correlated well with the maximal rate of cell death of the androgen-dependent prostatic epithelial cells. These results suggest a temporal correlation between the expression of TGF.beta. transcripts and the activation of programmed cell death in the rat ventral prostate after castration-induced androgen withdrawal.