Characterization of a novel β-cypermethrin-degrading Aspergillus niger YAT strain and the biochemical degradation pathway of β-cypermethrin
Characterization of a novel β-cypermethrin-degrading Aspergillus niger YAT strain and the biochemical degradation pathway of β-cypermethrin
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新型高效氯氰菊酯降解黑曲霉 YAT 菌株的鉴定及高效氯氰菊酯的生化降解途径
DOI：
10.1007/s00253-015-6690-2
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发表时间：
2015-10-01
影响因子：
5
通讯作者：
Liu, Shuliang
中科院分区：
文献类型：
--
作者：
Deng, Weiqin;Lin, Derong;Liu, Shuliang
从中国砖茶（保藏号：CGMCC 10, 568）中分离到一株黑曲霉（Aspergillusniger）YAT菌株，通过形态学特征和ITS序列分析对其进行鉴定。该菌株对高效氯氰菊酯（beta-CY; 50 mg L-1）的降解率为54.83%，对3-苯氧基苯甲酸（3-PBA; 100 mg L-1）的降解率为100%。β-CY和3-PBA的半衰期分别为3.573 - 11.748天和5.635 - 12.160 h。采用一级动力学模型进一步描述了β-CY和3-PBA的降解。通过高效液相色谱（HPLC）和液相色谱-质谱（LC-MS）联用技术分析降解产物，探讨YAT降解β-CY的途径和机理。相关的酶活性和底物利用率也进行了研究。分析β-CY降解产物。结果表明，YAT菌株能将β-CY转化为3-PBA。3-PBA在降解过程中逐渐转化为二甲酸、原儿茶酸、3-羟基-5-苯氧基苯甲酸、没食子酸和苯酚。YAT菌株还可以有效地降解这些代谢产物。结果表明，YAT菌株在拟除虫菊酯类农药污染环境和发酵食品的生物修复中具有潜在的应用价值。
Aspergillus niger YAT strain was obtained from Chinese brick tea (Collection number: CGMCC 10,568) and identified on the basis of morphological characteristics and internal transcribed spacer (ITS) sequence. The strain could degrade 54.83 % of beta-cypermethrin (beta-CY; 50 mg L-1) in 7 days and 100 % of 3-phenoxybenzoic acid (3-PBA; 100 mg L-1) in 22 h. The half-lives of beta-CY and 3-PBA range from 3.573 to 11.748 days and from 5.635 to 12.160 h, respectively. The degradation of beta-CY and 3-PBA was further described using first-order kinetic models. The pathway and mechanism of beta-CY degraded by YAT were investigated by analyzing the degraded metabolites through high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Relevant enzymatic activities and substrate utilization were also investigated. beta-CY degradation products were analyzed. Results indicated that YAT strain transformed beta-CY into 3-PBA. 3-PBA was then gradually transformed into permethric acid, protocatechuic acid, 3-hydroxy-5-phenoxy benzoic acid, gallic acid, and phenol gradually. The YAT strain can also effectively degrade these metabolites. The results indicated that YAT strain has potential applications in bioremediation of pyrethroid insecticide (PI)-contaminated environments and fermented food.