MYB transcription factor PdMYB118 directly interacts with bHLH transcription factor PdTT8 to regulate wound-induced anthocyanin biosynthesis in poplar
MYB transcription factor PdMYB118 directly interacts with bHLH transcription factor PdTT8 to regulate wound-induced anthocyanin biosynthesis in poplar
复制标题
MYB转录因子PdMYB118直接与bHLH转录因子PdTT8相互作用调节杨树伤口诱导的花青素生物合成
DOI：
10.1186/s12870-020-02389-1
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发表时间：
2020-04-20
影响因子：
5.3
通讯作者：
Zhang, Hongxia
中科院分区：
文献类型：
--
作者：
Wang, Haihai;Wang, Xiaoqing;Zhang, Hongxia
研究背景R2 R3-MYB转录因子在植物的生长发育和对生物和非生物胁迫的应答中起着重要作用。然而，它们在创伤诱导的木本植物花青苷生物合成中的调控机制在很大程度上是未知的。结果在这项工作中，我们报告了花青苷生物合成基因（ABG）的表达被美洲黑杨MYB TF编码基因PdMYB 118激活，PdMYB 118的激活被bHLH蛋白PdTT 8显著增强，PdMYB 118和一些ABG在创伤诱导和茉莉酸甲酯（MeJA）处理中有明显的诱导作用。PdMYB 118的过表达促进了转基因白杨愈伤组织中花青苷的积累。此外，白杨JASMONATE ZIM结构域（JAZ）蛋白PtrJAZ 1通过与PdTT 8结合抑制PdMYB 118/PdTT 8复合体的转录功能，并刺激茉莉酸（JA）的生物合成和PtrJAZ 1的降解。导致转基因白杨受伤叶片中花青素苷的生物合成增加。因此，我们的研究结果不仅阐明了PdMYB 118在白杨创伤诱导花色素苷合成中的重要作用，而且为彩色树种的基因工程提供了分子基础。
BackgroundR2R3-MYB transcription factors (TFs) play important roles in plant growth and development, and response to biotic and abiotic stresses. However, their regulatory mechanisms in wound-induced anthocyanin biosynthesis in woody plants are largely unknown.ResultsIn this work, we report that expression of anthocyanin biosynthesis genes (ABGs) were activated by PdMYB118, a MYB TF encoding gene fromPopulus deltoids, and the activation of PdMYB118 was significantly enhanced by PdTT8, a bHLH protein, through its direct interaction with PdMYB118.PdMYB118and someABGswere evidently induced by wound induction and methyl jasmonate (MeJA) treatment. Overexpression ofPdMYB118promoted anthocyanin accumulation in transgenic poplar upon wound induction. Furthermore, a poplar JASMONATE ZIM-domain (JAZ) protein, PtrJAZ1, repressed the transcriptional function of PdMYB118/PdTT8 complex by binding to PdTT8, and wound stimulated the biosynthesis of jasmonic acid (JA) and the degradation of PtrJAZ1.ConclusionsBased on these observations, we proposed that PtrJAZ1 degradation triggered the expression ofABGs, leading to increased biosynthesis of anthocyanins in the wounded leaves of transgenic poplar. Therefore, our findings not only illustrate the crucial role of PdMYB118 in wound-induced anthocyanin biosynthesis in poplar, but also provide a molecular basis for the genetic engineering of colorful tree species.