Cation channels in the plasma membrane of rye roots.
Cation channels in the plasma membrane of rye roots.
复制标题
黑麦根质膜中的阳离子通道。
DOI：
10.1093/jxb/48.special_issue.499
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发表时间：
1997
影响因子：
6.9
通讯作者：
Philip J. White
中科院分区：
文献类型：
--
作者：
Philip J. White
通过表皮细胞的膜片钳原生质体在体内和体外将质膜囊泡重建为平面脂质双层 (PLB)，对黑麦 (Secale Greeke L.) 根细胞质膜中的电压依赖性阳离子通道进行了电学研究。膜片钳技术识别出一个时间相关的内向整流 K(+) 电流和两个时间相关的外向整流电流，其中一个对 K(+) 具有相对选择性，而另一个在阳离子之间没有选择性。此外，在膜片钳记录中观察到与时间无关的K(+)电流。这种电流被认为是由电压无关的通道携带的，该通道可渗透单价阳离子，并且其生物物理特性与 PLB 中最常见的通道的生物物理特性相对应。在 PLB 中经常观察到另外两个阳离子通道。这些通道可渗透单价和二价阳离子，并通过质膜去极化激活。它们可能在细胞内信号传导中发挥作用，因为它们会介导 Ca(2+) 内流以响应体内质膜去极化。本文讨论了每个阳离子通道的生物物理特性和生理作用，并与对比细胞类型和植物物种的根细胞质膜中的阳离子通道进行了比较。
Voltage-dependent cation channels in the plasma membrane of rye (Secale cereale L.) root cells have been studied electrically both in vivo, by patch-clamping protoplasts of epidermal cells, and in vitro, following the reconstitution of plasma-membrane vesicles into planar lipid bilayers (PLB). The patch-clamp technique has identified a time-dependent inward-rectified K(+) current and two time-dependent outward-rectified currents, one of which is relatively selective for K(+) whilst the other is non-selective between cations. In addition, a time-independent K(+)-current has been observed in patch-clamp recordings. This current is believed to be carried by voltage-independent channels, which are permeable to monovalent cations and whose biophysical properties correspond to those of the channel most frequently observed in PLB. Two further cation channels are frequently observed in PLB. These channels are permeable to both monovalent and divalent cations and are activated by plasmamembrane depolarization. They may have a role in intracellular signalling since they would mediate Ca(2+) influx in response to plasma-membrane depolarization in vivo. In this paper the biophysical properties and physiological roles of each cation channel are discussed and compared with cation channels in the plasma membrane of root cells from contrasting cell types and plant species.
影响因子：
11.6
作者：
CAO, YW;ANDEROVA, M;SCHROEDER, JI
通讯作者：
SCHROEDER, JI