Fabrication and modification of implantable optrode arrays for in vivo optogenetic applications.
Fabrication and modification of implantable optrode arrays for in vivo optogenetic applications.
复制标题
用于体内光遗传学应用的植入式光极阵列的制造和修改
DOI：
10.1007/s41048-018-0052-4
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发表时间：
2018
影响因子：
--
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Wang L;Huang K;Zhong C;Wang L;Lu Y
图解摘要摘要光遗传学的最新进展已经建立了一种精确定时和细胞特异性的方法来理解大脑回路的功能和神经精神疾病的机制。然而，光极（光遗传学中的关键功能元件）的制造仍然是一个巨大的挑战。在这里，我们报告了长期植入光极阵列的可靠且高效的制造策略。为了提高制造的光极阵列的性能，使用优化的电化学过程对记录位点的表面进行了修改。我们还证明了使用制造的光极阵列检测多个大脑区域的癫痫发作并抑制体内发作传播的可行性。此外，组织学研究的结果表明，复合导电聚合物的电沉积显着减轻了植入物/神经组织界面的炎症反应并提高了神经元的存活率。总之，我们为定制光极阵列的制造和修改提供了可靠且有效的策略，可以满足体内光遗传学应用的要求。
Graphical AbstractAbstractRecent advances in optogenetics have established a precisely timed and cell-specific methodology for understanding the functions of brain circuits and the mechanisms underlying neuropsychiatric disorders. However, the fabrication of optrodes, a key functional element in optogenetics, remains a great challenge. Here, we report reliable and efficient fabrication strategies for chronically implantable optrode arrays. To improve the performance of the fabricated optrode arrays, surfaces of the recording sites were modified using optimized electrochemical processes. We have also demonstrated the feasibility of using the fabricated optrode arrays to detect seizures in multiple brain regions and inhibit ictal propagation in vivo. Furthermore, the results of the histology study imply that the electrodeposition of composite conducting polymers notably alleviated the inflammatory response and improved neuronal survival at the implant/neural-tissue interface. In summary, we provide reliable and efficient strategies for the fabrication and modification of customized optrode arrays that can fulfill the requirements of in vivo optogenetic applications.