ACID MUCOPOLYSACCHARIDE (GLYCOSAMINOGLYCAN) AT THE EPITHELIAL-MESENCHYMAL INTERFACE OF MOUSE EMBRYO SALIVARY GLANDS
ACID MUCOPOLYSACCHARIDE (GLYCOSAMINOGLYCAN) AT THE EPITHELIAL-MESENCHYMAL INTERFACE OF MOUSE EMBRYO SALIVARY GLANDS
复制标题
小鼠胚胎唾液腺上皮间质界面的酸性粘多糖（糖胺聚糖）
作者：
M. Bernfield;S. Banerjee
酸性粘多糖（糖胺聚糖）已在小鼠胚胎下颌下腺的上皮 - 间质界面通过以下方法得到证实：（a）在不同镁浓度下用阿尔辛蓝8 GX对聚合硫酸酯进行特异性染色，（b）通过将这些残基选择性氧化为席夫反应性化合物对聚合糖醛酸进行特异性染色，（c）钌红染色的电子显微镜定位，（d）葡糖胺 - ³H和³⁵SO₄的放射自显影定位，以及（e）界面处葡糖胺放射性对无蛋白酶透明质酸酶消化的敏感性。此外，从腺体中分离出了用葡糖胺 - ³H和³⁵SO₄标记且化学特性与酸性粘多糖相同的物质。酸性粘多糖分布在整个上皮表面。染色过程显示，酸性粘多糖的量在所有部位几乎相等。相比之下，在生长和分支的小叶远端表面，葡糖胺标记的粘多糖的积累速率更高。新合成的酸性粘多糖在初始裂隙形成部位的这种分布表明，表面相关的酸性粘多糖参与形态发生过程。提出了一种分支形态发生机制，该机制解释了胶原纤维以及上皮表面的总酸性粘多糖和新合成的酸性粘多糖的分布。
Acid mucopolysaccharide (glycosaminoglycan) has been demostrated at the epithelial-mesenchymal interface of mouse embryo submandibular glands by (a) specific staining for polymeric sulfate with Alcian blue 8 GX at various magnesium concentrations, (b) specific staining for polymeric uronic acid by selective oxidation of these residues to Schiff-reactive compounds, (c) electron microscope localization of ruthenium red staining, (d) radioautographic localization of glucosamine-3H and 35SO4, and (e) by susceptibility of the glucosamine radioactivity at the interface to digestion with protease-free hyaluronidase. Moreover, material labeled with glucosamine-3H and 35SO4 and with chemical characteristics identical with those of acid mucopolysaccharide were isolated from the glands. Acid mucopolysaccharide is distributed over the entire epithelial surface. The amount of acid mucopolysaccharide, as revealed by the staining procedures, is nearly equivalent at all sites. In contrast, the rate of accumulation of glucosamine-labeled mucopolysaccharide is greater at the surface of the distal ends of the growing and branching lobules. This distribution of newly synthesized acid mucopolysaccharide at the sites of incipient cleft formation suggests that surface-associated acid mucopolysaccharide is involved in the morphogenetic process. A mechanism of branching morphogenesis is proposed which accounts for the distribution of collagen fibers and total and newly synthesized acid mucopolysaccharide at the epithelial surface.