Synergistic effects of enamel matrix derivatives and surface morphology of anodized titanium on osteogenic differentiation of bone marrow mesenchymal stem cells sheet
Synergistic effects of enamel matrix derivatives and surface morphology of anodized titanium on osteogenic differentiation of bone marrow mesenchymal stem cells sheet
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牙釉质基质衍生物与阳极氧化钛表面形态对骨髓间充质干细胞片成骨分化的协同作用
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发表时间：
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影响因子：
2.2
通讯作者：
Qiang Dong
中科院分区：
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作者：
Ying Li;Lu Cheng;Qian Xia;MaoHua Meng;QinYing Wang;Xiao Zeng;YuanYuan Jia;ChunYing Liu;Lei Wu;Helin Chen;Qiang Dong
摘要：目的：探讨釉基质衍生物（EMD）在不同钛表面形态的骨髓间充质干细胞（BMSC）片上诱导成骨的可能机制。研究方法：将骨髓基质细胞接种于阳极氧化、喷砂、粗砂和酸蚀3种不同形貌的钛合金表面，并进行对照。在相同浓度的EMDs作用下，观察不同表面形态的BMSCs的增殖和成骨分化情况。为了进一步了解EMD在不同形态的BMSC片上的成骨机制，采用实时荧光定量RT-PCR和western blot检测成骨基因和成骨蛋白的表达水平。最后，将不同形态的BMSC片植入裸鼠背部皮下，HE染色检测成骨情况，以验证经EMD刺激的BMSC片在体内的成骨作用。结果如下：AO组的EMDs和表面形态学协同提高了成骨活性因子RUNX 2、OSX和OCN的表达水平，增强了BMSCs的成骨分化效果。体内实验表明，AO组BMSCs片层富含成骨活性因子，植入裸鼠背部皮下后促进异位骨组织形成。结论：EMDs和AO形态学协同增强BMSCs骨成骨活性因子的分泌，促进异位骨形成。
Abstract: Objective: To explore the potential osteogenic induction mechanism of enamel matrix derivatives (EMDs) on bone marrow mesenchymal stem cell (BMSC) sheets with different titanium surface morphologies. Methods: The BMSCs were inoculated on the surfaces of titanium alloys with three different morphologies: anodic oxidation (AO), sand-blasted, large grit and acid-etched , and no treatment (control). The proliferation and osteogenic differentiation of BMSCs on the different surface morphologies were observed with the same concentration of EMDs. In order to further understand the osteogenic mechanism of EMDs on BMSC sheets with different morphologies, a real-time RT-PCR and a western blot were used to detect the overall levels of osteogenic genes and osteogenic proteins. Finally, to verify the osteogenic effect of BMSC sheets stimulated by EMDs in vivo, BMSC sheets with different morphologies were implanted into the subcutaneous tissue of the back of nude mice, and the bone formation was detected by HE staining. Results: The EMDs and surface morphology in the AO group synergically increased the expression levels of osteogenic active factors (RUNX2, OSX and OCN) and enhanced the osteogenic differentiation effect of BMSCs. The in vivo experiments showed that the BMSCs sheet in the AO group, rich in osteogenic active factors, promoted the formation of ectopic bone tissue after implantation into the subcutaneous tissue of the back of nude mice. Conclusion: EMDs and AO morphology synergically enhance the secretion of bone osteogenic active factors of BMSCs and promote the formation of heterotopic bone.