SEQUENCE-SPECIFIC RECOGNITION OF THE MAJOR GROOVE OF DNA BY OLIGODEOXYNUCLEOTIDES VIA TRIPLE HELIX FORMATION - FOOTPRINTING STUDIES
SEQUENCE-SPECIFIC RECOGNITION OF THE MAJOR GROOVE OF DNA BY OLIGODEOXYNUCLEOTIDES VIA TRIPLE HELIX FORMATION - FOOTPRINTING STUDIES
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DOI：
10.1093/nar/16.24.11431
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发表时间：
1988-12-23
影响因子：
14.9
通讯作者：
HELENE, C
中科院分区：
文献类型：
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作者：
FRANCOIS, JC;SAISONBEHMOARAS, T;HELENE, C
同型嘧啶寡脱氧核苷酸通过形成局部三螺旋来识别同型嘌呤、同型嘧啶序列的DNA双螺旋的主沟。寡核苷酸与双链体的高嘌呤链平行结合。这种结合可以通过使用铜菲咯啉作为裂解试剂的足迹技术来揭示。寡核苷酸结合在大沟中可防止铜菲咯啉的裂解。三螺旋边界处双链体相对链上的裂解模式是不对称的。它们转移到 3'' 侧，表明铜-菲咯啉螯合物结合在双链体结构的小沟中。螯合物在三螺旋和双螺旋之间的连接处的结合在结合的同嘧啶寡核苷酸的5''侧上不受干扰。相反，在同嘧啶寡核苷酸3''侧的三链体-双链体连接处的含嘌呤链上观察到切割的强烈增强。
Homopyrimidine oligodeoxynucleotides recognize the major groove of the DNA double helix at homopurine, homopyrimidine sequences by forming local triple helices. The oligonucleotide is bound parallel to the homopurine strand of the duplex. This binding can be revealed by a footprinting technique using copper-phenanthroline as a cleaving reagent. Oligonucleotide binding in the major groove prevents cleavage by copper-phenanthroline. The cleavage patterns on opposite strands of the duplex at the boundaries of the triple helix are asymmetric. They are shifted to the 3''-side, indicating that the copper-phenanthroline chelate binds in the minor groove of the duplex structure. Binding of the chelate at the junction between the triple and the double helix is not perturbed on the 5''-side of the bound homopyrimidine oligonucleotide. In contrast, a strong enhancement of cleavage is observed on the purine-containing strand at the triplex-duplex junction on the 3''-side of the homopyrimidine oligonucleotide.