The mitochondrial genome of spotted green pufferfish Tetraodon nigroviridis (Teleostei: Tetraodontiformes) and divergence time estimation among model organisms in fishes
The mitochondrial genome of spotted green pufferfish Tetraodon nigroviridis (Teleostei: Tetraodontiformes) and divergence time estimation among model organisms in fishes
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DOI：
10.1266/ggs.81.29
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发表时间：
2006-02-01
影响因子：
1.1
通讯作者：
Nishida, Mutsumi
中科院分区：
文献类型：
--
作者：
Yamanoue, Yusuke;Miya, Masaki;Nishida, Mutsumi
我们测定了黑斑河豚(Tetraodon Nigroviridis)线粒体基因组的全序列。该基因组全长16,488bp，包含37个基因(2个核糖体RNA基因、22个转移RNA基因和13个蛋白质编码基因)以及与其他脊椎动物相同的控制区，基因序列与典型脊椎动物相同。该序列被用来估计鱼类主要谱系之间的系统发育关系和分化时间，包括鱼类中具有代表性的模式生物。我们使用分割贝叶斯方法进行这两个分析，使用的两个数据集包括来自12个蛋白质编码基因(不包括ND6基因)的串联氨基酸序列和来自12个蛋白质编码基因(没有第三个密码子位置)、22个转移RNA基因和两个核糖体RNA基因的串联核苷酸序列。从两个数据集得到的树被很好地分辨出来，并与之前的研究结果基本一致，斑点绿色河豚被放置在一个合理的系统发育位置。斑点绿河豚与鱼类模式生物之间的大致分歧时间为8500万年前(MYA)对龙舌鱼、183 MYA对三刺刺鱼、191 MYA对青竹、324 MYA对斑马鱼，所有这些都是根据化石记录中最早出现的斑马鱼估计的分歧时间的两倍。
We determined the whole mitochondrial genome sequence for spotted green pufferfish, Tetraodon nigroviridis (Teleostei: Tetraodontiformes). The genome (16,488 bp) contained 37 genes (two ribosomal RNA genes, 22 transfer RNA genes, and 13 protein-coding genes) plus control region as found in other vertebrates, with the gene order identical to that of typical vertebrates. The sequence was used to estimate phylogenetic relationships and divergence times among major lineages of fishes, including representative model organisms in fishes. We employed partitioned Bayesian approaches for these two analyses using two datasets that comprised concatenated amino acid sequences from 12 protein-coding genes (excluding the ND6 gene) and concatenated nucleotide sequences from the 12 protein-coding genes (without 3rd codon positions), 22 transfer RNA genes, and two ribosomal RNA genes. The resultant trees from the two datasets were well resolved and largely congruent with those from previous studies, with spotted green pufferfish being placed in a reasonable phylogenetic position. The approximate divergence times between spotted green pufferfish and model organisms in fishes were 85 million years ago (MYA) vs. torafugu, 183 MYA vs. three-spined stickleback, 191 MYA vs. medaka, and 324 MYA vs. zebrafish, all of which were about twice as old as the divergence times estimated by their earliest occurrences in fossil records.