HPLC analysis of Juzen-taiho-to and its variant formulations and their antimetastatic efficacies
HPLC analysis of Juzen-taiho-to and its variant formulations and their antimetastatic efficacies
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DOI：
10.1248/cpb.47.1170
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发表时间：
1999-08-01
影响因子：
1.7
通讯作者：
Nunome, S
中科院分区：
文献类型：
--
作者：
Saiki, I;Yamaura, T;Nunome, S
我们以前的研究表明，与未处理的对照相比，口服Juzen-taiho-to导致结肠26-L5细胞肝转移的显著抑制，没有副作用。我们试图研究HPLC图谱之间的关系为了获得制剂的最佳功效和恒定的质量，两种Juzen-taiho-to制剂使用相同的10种原料药和相同的制备方法单独制备的两种化合物（批次#1和#2）通过HPLC分析显示出相似的抗转移作用和吸光度模式。Juzen-taiho-to的一些变体制剂，其中一种原料药被来自不同来源或产地的其他原料药取代，与原始制剂相比，表现出降低的功效，以及与原始制剂相比指纹图谱的差异。瑞曾用不同产地和不同采收地的甘草替代汉方（Naimo-Ogi-Kibana-Ogi），对肿瘤细胞肝转移有明显的抑制作用，其HPLC指纹图谱与原方相似。因此，汉方药物的HPLC指纹图谱分析可为获得其最佳疗效和稳定制剂质量提供有用的依据。但也存在一些问题和局限性，如对紫外吸收的检测能力和敏感性。
Our previous study demonstrated that the oral administration of Juzen-taiho-to resulted in a significant inhibition of the liver metastasis of colon 26-L5 cells as compared with the untreated control, without side effects. We attempted to investigate the relationship between the HPLC pattern (referred to as the fingerprint) of the formulation and its component crude drugs and the inhibition of tumor metastasis in order to obtain the optimal efficacy and constant quality of the formulation, Two Juzen-taiho-to formulations (batches #1 and # 2),,which were individually prepared using the same 10 crude drugs and the same preparation procedure, showed similar anti-metastatic effects and absorbance patterns by HPLC analysis. Some variant formulations of Juzen-taiho-to, in which one crude drug was substituted with other crude drugs from different sources or places of origin, exhibited reduced efficacy as compared with the original formulation, as well as differences in the fingerprint pattern compared with the original formulation. Juzen (Naimo-Ogi-Kibana-Ogi), a variant formulation with the substitution of Astragali radix of a different origin and place of harvest, showed significant inhibition of the liver metastasis of tumor cells and a HPLC fingerprint pattern similar to that of the original formulation, Thus, HPLC fingerprint analysis of Kampo medicines may provide a useful basis for obtaining their optimal efficacy as well as constant quality of the formulation, although it has some problems and limitations, such as detectability by and senitivity to UV absorbance.