SPATIAL AND TEMPORAL DISSECTION OF IMMEDIATE AND EARLY EVENTS FOLLOWING CADHERIN-MEDIATED EPITHELIAL-CELL ADHESION
SPATIAL AND TEMPORAL DISSECTION OF IMMEDIATE AND EARLY EVENTS FOLLOWING CADHERIN-MEDIATED EPITHELIAL-CELL ADHESION
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DOI：
10.1083/jcb.120.5.1217
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发表时间：
1993-03-01
影响因子：
7.8
通讯作者：
NELSON, WJ
中科院分区：
文献类型：
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作者：
MCNEILL, H;RYAN, TA;NELSON, WJ
细胞-细胞黏附是导致上皮细胞结构和功能重塑的蛋白质相互作用的分子级联反应的顶端。人们对引发这一连锁效应的最早事件知之甚少。利用高分辨率差分干涉显微镜和回顾性免疫组织化学方法，我们观察到MDCK上皮细胞的细胞-细胞接触由不同的阶段组成，这些阶段与E-钙粘蛋白与细胞骨架相互作用的特定变化有关。我们表明，在形成稳定的接触之前，有大量的瞬时接触。在这段时间内以及在形成稳定的接触后，接触处E-钙粘素的分布、相对量或Triton X-100不溶性没有可检测到的变化。在大约10分钟的滞后期后，快速获得定位于稳定接触处的E-钙粘附素的Triton X-100不溶性。值得注意的是，在这段时间内，接触处的E-钙粘素总量保持不变。Triton X-100不溶性E-钙粘素池的增加与肌动蛋白或Fodrin分布的变化无关，这表明Triton X-100不溶性的获得是由于E-钙粘附素本身或密切相关的蛋白质(如连接素)的变化。10分钟的滞后期，以及随后E-钙粘素重组的即时性和局部性，表明某种形式的信号正在发生。
Cell-cell adhesion is at the top of a molecular cascade of protein interactions that leads to the remodeling of epithelial cell structure and function. The earliest events that initiate this cascade are poorly understood. Using high resolution differential interference contrast microscopy and retrospective immunohistochemistry, we observed that cell-cell contact in MDCK epithelial cells consists of distinct stages that correlate with specific changes in the interaction of E-cadherin with the cytoskeleton. We show that formation of a stable contact is preceded by numerous, transient contacts. During this time and immediately following formation of a stable contact, there are no detectable changes in the distribution, relative amount, or Triton X-100 insolubility of E-cadherin at the contact. After a lag period of approximately 10 min, there is a rapid acquisition of Triton X-100 insolubility of E-cadherin localized to the stable contact. Significantly, the total amount of E-cadherin at the contact remains unchanged during this time. The increase in the Triton X-100 insoluble pool of E-cadherin does not correlate with changes in the distribution of actin or fodrin, suggesting that the acquisition of the Triton X-100 insolubility is due to changes in E-cadherin itself, or closely associated proteins such as the catenins. The 10 minute lag period, and subsequent prompt and localized nature of E-cadherin reorganization indicate a form of signaling is occurring.