Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus
Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus
复制标题
DOI：
10.1128/jvi.71.1.734-737.1997
复制
发表时间：
1997-01-01
影响因子：
5.4
通讯作者：
Delmas, B
中科院分区：
文献类型：
--
作者：
Benbacer, L;Kut, E;Delmas, B
Ne报道，当用含有残基643和841之间的犬结构域的嵌合氨基肽酶-N（APN）cDNA转染时，对犬冠状病毒（CCV）和猫传染性腹膜炎病毒（FIPV）不敏感的细胞变得敏感。这一发现表明，APN识别这些病毒是物种相关的，并与此C-末端结构域。人/犬APN嵌合体也能够赋予对猪传染性胃肠炎病毒（TGEV）的易感性，而其人/猪同源物未能赋予对CCV和FIPV的易感性。观察到CCV、FIPV和TGEV识别不同种间APN嵌合体的能力与它们感染来自相关种的细胞的能力之间存在良好的相关性。作为例外，发现TGEV使用人/牛APN嵌合体作为受体，尽管其本身不能在牛细胞中复制。
Ne report that cells refrectory to canine coronavirus (CCV) and feline infectious peritonitis virus (FIPV) became susceptible when transfected with a chimeric aminopeptidase-N (APN) cDNA containing a canine domain between residues 643 and 841. This finding shows that APN recognition by these viruses is species related and associated with this C-terminal domain. The human/canine APN chimera was also able to confer susceptibility to the porcine transmissible gastroenteritis virus (TGEV), whereas its human/porcine homolog failed to confer susceptibility to CCV and FIPV. A good correlation was observed between the capacity of CCV, FIPV, and TGEV to recognize the different interspecies APN chimeras and their ability to infect cells derived from the relevant species. As an exception, TGEV was found to use a human/bovine APN chimera as a receptor although itself unable to replicate in bovine cells.