Real-Time Imaging Tracking of Mesenchymal Stem Cells Labeled with Lipid-Poly(lactic-co-glycolic acid) Nanobubbles
Real-Time Imaging Tracking of Mesenchymal Stem Cells Labeled with Lipid-Poly(lactic-co-glycolic acid) Nanobubbles
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脂质聚乳酸乙醇酸纳米气泡标记的间充质干细胞的实时成像跟踪
DOI：
10.1166/jbn.2019.2852
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发表时间：
2019
影响因子：
2.9
通讯作者：
Yan Fei
中科院分区：
文献类型：
--
作者：
Liu Hongmei;Liu Sai;Liang Yangbiao;Deng Zhiting;Wang Yu;Yan Fei
基于干细胞的治疗由于对经历各种疾病和损伤的患者的显著益处而引起了相当大的关注。然而，其潜在的作用机制尚未完全了解。一个主要原因是缺乏实时跟踪深层移植干细胞的成像工具。在本研究中，我们开发了一种具有纳米尺寸、良好相容性和强对比增强超声信号的脂质聚（乳酸-羟基乙酸）纳米泡（LPN）探头。由于纳米级颗粒尺寸，间充质干细胞的细胞标记可以通过与LPN孵育来实现。这些标记的干细胞在体外和体内获得显着增强的超声图像。更重要的是，标记的干细胞可以通过超声成像跟踪长达5天。额外的评价发现，体内检测限达到了活小鼠皮下组织中2，000个标记干细胞。我们的研究提出了一种实现实时超声成像跟踪的策略，为研究干细胞治疗的潜在机制和未来的临床应用铺平了道路。
Stem-cell-based therapy has attracted considerable attention due to the significant benefits to patients experiencing a wide range of diseases and injuries. However, their underlying mechanism of action is not completely understood. One main reason is the lack of imaging tools for real-time tracking of deep-seated transplanted stem cells. For the present study, we exploited a lipid poly(lactic-co-glycolic acid) nanobubble (LPN) probe with nanoscale size, good compatibility, and strong contrast-enhanced ultrasound signals. Due to the nanoscale particle size, cellular labeling of mesenchymal stem cells can be achieved via incubation with LPNs. Significantly enhanced ultrasound images of these labeled stem cells were obtained in vitro and in vivo. More importantly, the labeled stem cells could be tracked by ultrasound imaging for up to 5 days. Additional evaluation found that the in vivo detection limit achieved 2,000 labeled stem cells in the subcutaneous tissues of living mice. Our study presents a strategy to achieve real-time ultrasound imaging tracking, paving the way for an investigation on the underlying mechanism and future clinical application of stem cell therapy.