Role of gastric mucosal constitutive and inducible nitric oxide synthases in the development of stress-induced gastric mucosal lesions in rats.
Role of gastric mucosal constitutive and inducible nitric oxide synthases in the development of stress-induced gastric mucosal lesions in rats.
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DOI：
10.1006/bbrc.1997.6972
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发表时间：
1997-07
影响因子：
3.1
通讯作者：
K. Nishida;Y. Ohta;I. Ishiguro
中科院分区：
文献类型：
--
作者：
K. Nishida;Y. Ohta;I. Ishiguro
本实验观察了一氧化氮合酶（NOS）抑制剂预处理和未预处理的大鼠胃黏膜中组成型一氧化氮合酶（cNOS）和诱导型一氧化氮合酶（iNOS）活性随水浸束缚（WIR）应激所致胃黏膜损伤发展的变化。随着胃粘膜病变的发展，胃粘膜中cNOS活性逐渐降低，iNOS活性逐渐升高。非选择性NOS抑制剂N（G）-monomethyl L-arginine预处理后，胃粘膜cNOS和iNOS活性均受到抑制，但仍高于正常水平。用选择性iNOS抑制剂氨基胍预处理，通过抑制胃粘膜中iNOS活性和维持cNOS活性来防止胃粘膜损伤的发展。这些结果表明，在WIR应激大鼠中，胃粘膜中iNOS活性的增加和cNOS活性的降低与胃粘膜病变的发展密切相关。
Changes in gastric mucosal constitutive NO synthase (cNOS) and inducible NO synthase (iNOS) activities with the development of gastric mucosal lesions induced by water immersion restraint (WIR) stress were investigated in rats pretreated with and without NOS inhibitors. A decrease in cNOS activity and an increase in iNOS activity in the gastric mucosa occurred with gastric mucosal lesion development. Pretreatment with N(G)-monomethyl L-arginine, a non-selective NOS inhibitor, enhanced gastric mucosal lesion development with inhibition of gastric mucosal cNOS and iNOS activities, although the inhibited iNOS activity was still higher than the normal level. Pretreatment with aminoguanidine, a selective iNOS inhibitor, prevented gastric mucosal lesion development with inhibition of iNOS activity and maintenance of cNOS activity in the gastric mucosa. These results indicate that in WIR-stressed rats, an increase in iNOS activity and a decrease in cNOS activity in the gastric mucosa are closely related to the development of gastric mucosal lesions.