Second-harmonic microscopy of unstained living cardiac myocytes: measurements of sarcomere length with 20-nm accuracy
Second-harmonic microscopy of unstained living cardiac myocytes: measurements of sarcomere length with 20-nm accuracy
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DOI：
10.1364/ol.29.002031
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发表时间：
2004-09-01
期刊：
影响因子：
3.6
通讯作者：
Schanne-Klein, MC
中科院分区：
文献类型：
--
作者：
Boulesteix, T;Beaurepaire, E;Schanne-Klein, MC
我们将二次谐波发生 (SHG) 显微镜扩展到未染色的活体心肌细胞中肌节长度的测量，精度为 20 nm。我们量化了石房蛤毒素存在下个体肌节的缩短，发现它与心房组织挛缩的机械测量结果一致。 SHG 显微镜的这种功能应用通常适用于量化药物对收缩组织的生理效应。我们的数据还表明，肌节粗丝中堆积的肌球蛋白头负责肌肉组织中的大二次谐波内源信号。 (C) 2004 年美国光学学会。
We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue. (C) 2004 Optical Society of America.