Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis
Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis
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DOI：
10.1023/a:1023062316439
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发表时间：
2003-04
期刊：
影响因子：
1.9
通讯作者：
Yueming Yan;S. Hsam;Jianzhong Yu;Yi Jiang;F. Zeller
中科院分区：
文献类型：
--
作者：
Yueming Yan;S. Hsam;Jianzhong Yu;Yi Jiang;F. Zeller
Variability of high molecular weight glutenin subunits (HMW-GS) was studied in198 accessions ofAe. Tauschii(2n=2x=14, DD) by sodium dodecyl sulphate(SDS-PAGE) and acid polyacrylamide gel electrophoresis (A-PAGE) and capillary electrophoresis (CE). A high allelic variation of HMW-GS, including some novel x- and y-type subunits and variable subunit combinations were observed. One accession(TD159) showed a x-type null form. The results by A-PAGE analysis revealed that the subunitsDx5tandDy10tencoded byGlu-Dt1locus inAe. tauschiiwere different in relative mobilities in comparison with the subunits Dx5 and Dy10 found in bread wheats, whereas they had the same mobilities, respectively, when separated by SDS-PAGE. The higher resolution ofAe. tauschiiHMW-GS separated by CE method showed two clear peaks in accordance with x- and y-type subunits, respectively,except the accession TD151 which possessed only subunit Dy12.1*t. The electro elution time of the x-type and y-type subunits were about 13–14 and 7–8minutes, respectively. Characterization of wheat HMW-GS was facilitated by using CE which provides high resolution and increases the speed of analysis in conjunction with the traditional gel electrophoretic methods. A total of 42HMW-GS alleles were identified, among which were several alleles not presently detected in bread wheats. HenceAe. tauschiiis potentially a valuable genetic resource for quality improvement of bread wheat.
