Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium
Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium
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DOI：
10.1007/s00792-008-0194-2
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发表时间：
2009-01-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Shuang-Jiang
中科院分区：
文献类型：
--
作者：
Cui, Heng-Lin;Zhou, Pei-Jin;Liu, Shuang-Jiang
对已有效公布名称的Haloarcula属和Halomicrobium属的所有成员进行了16S rRNA基因多态性调查，并研究了两个物种在不同NaCl浓度下生长过程中基因的转录。 Haloarcula和Halomicrobium物种至少具有两个不同的16S rRNA基因拷贝，并获得了18个新的16S rRNA基因序列。 Haloarcula 的 I 型和 II 型 16S rRNA 基因在 4.8-5.6% 的核苷酸位置上存在差异。 Halomicrobium mukohataei JCM 9738(T) 的 III 型和 IV 型 16S rRNA 基因具有 9.0% 的分歧，这是迄今为止所见的种内分歧最高的 16S rRNA 基因。基于16S rRNA基因的系统发育分析表明，所有I型16S rRNA基因都聚集在一起，Haloarcula物种的II型16S rRNA基因也是如此。分别由 I 型和 II 型 16S rRNA 基因产生的两个簇明显分离，它们的差异 (4.8-5.6%) 在盐杆菌目属之间通常发现的各种差异范围内 (约 5-10%)。逆转录PCR结果显示Har的I型和II型拷贝。 amylolytica BD-3(T) 以及 Hmc 的 III 型和 IV 型拷贝。 mukohataei JCM 9738(T)在不同盐浓度(15-28% NaCl)下均转录为16S rRNA分子。
All members of the genera Haloarcula and Halomicrobium whose names have been validly published were surveyed for 16S rRNA gene polymorphism, and the transcription of the genes from two species was investigated during growth at different NaCl concentrations. The species of Haloarcula and Halomicrobium harbour at least two different 16S rRNA gene copies, and 18 new sequences of 16S rRNA genes were obtained. The type I and type II 16S rRNA genes of Haloarcula are divergent at 4.8-5.6% of their nucleotide positions. The type III and type IV 16S rRNA genes from Halomicrobium mukohataei JCM 9738(T) are 9.0% divergent, which represents the highest intraspecific divergent 16S rRNA genes so far seen. Phylogenetic analysis based on 16S rRNA genes indicated that all type I 16S rRNA genes were clustered, and the same was true for the type II 16S rRNA genes of Haloarcula species. The two clusters, respectively generated from type I and type II 16S rRNA genes, were sharply separated and their divergences (4.8-5.6%) are in the range of various divergence usually found between genera in the order Halobacteriales (about 5-10%). Results from reverse transcription-PCR showed that the type I and type II copies of Har. amylolytica BD-3(T) and type III and type IV copies of Hmc. mukohataei JCM 9738(T) were all transcribed to 16S rRNA molecules under different salt concentrations (15-28% NaCl).