Oxidative Stickland reactions in an obligate aerobic organism - amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus.
Oxidative Stickland reactions in an obligate aerobic organism - amino acid catabolism in the Crenarchaeon Sulfolobus solfataricus.
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专性需氧生物中的氧化斯蒂克兰反应 - Crearchaeon Sulfolobus solfataricus 中的氨基酸分解代谢。
DOI：
10.1111/febs.14105
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Stark H
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文献类型：
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作者：
Stark H
嗜热嗜酸Crenarchaeon Sulfolobus solfataricus是古细菌适应极端环境的模式生物，以其降解多种底物的能力而闻名。它已经充分表征了许多碳水化合物作为碳源的利用。然而，它的氨基酸代谢，特别是单一氨基酸的降解，并没有得到很好的理解。在这项工作中，我们进行了代谢建模，以及代谢组学，转录组和蛋白质组学分析上生长的酪蛋白水解物作为碳源，以绘制一个全面的图片的氨基酸代谢在S.solfataricusP2。我们发现16种可检测的氨基酸中有10种是从生长培养基中输入的。总体而言，谷氨酸、蛋氨酸、亮氨酸、苯丙氨酸和异亮氨酸的摄取是所有观察到的氨基酸中最高的。我们的模拟预测亮氨酸和酪氨酸不完全降解为有机酸，并且根据这一点，我们检测到支链和芳香族有机酸以及氨基酸、铵和海藻糖输出到培养上清液中。支链氨基酸以及苯丙氨酸和酪氨酸通过氧化Stickland反应降解为有机酸。这种反应在能够厌氧生长的原核生物中是已知的，但迄今为止从未在专性需氧生物中观察到。此外，3-甲基-2-丁烯酸酯和2-甲基-2-丁烯酸酯首次被发现是用于降解支链氨基酸的改良Stickland反应的产物。这项工作首次详细描述了S. solfataricus中的支链和芳香族氨基酸catenoric。
The thermoacidophilic CrenarchaeonSulfolobus solfataricusis a model organism for archaeal adaptation to extreme environments and renowned for its ability to degrade a broad variety of substrates. It has been well characterised concerning the utilisation of numerous carbohydrates as carbon source. However, its amino acid metabolism, especially the degradation of single amino acids, is not as well understood. In this work, we performed metabolic modelling as well as metabolome, transcriptome and proteome analysis on cells grown on caseinhydrolysate as carbon source in order to draw a comprehensive picture of amino acid metabolism inS. solfataricusP2. We found that 10 out of 16 detectable amino acids are imported from the growth medium. Overall, uptake of glutamate, methionine, leucine, phenylalanine and isoleucine was the highest of all observed amino acids. Our simulations predict an incomplete degradation of leucine and tyrosine to organic acids, and in accordance with this, we detected the export of branched‐chain and aromatic organic acids as well as amino acids, ammonium and trehalose into the culture supernatants. The branched‐chain amino acids as well as phenylalanine and tyrosine are degraded to organic acids via oxidative Stickland reactions. Such reactions are known for prokaryotes capable of anaerobic growth, but so far have never been observed in an obligate aerobe. Also, 3‐methyl‐2‐butenoate and 2‐methyl‐2‐butenoate are for the first time found as products of modified Stickland reactions for the degradation of branched‐chain amino acids. This work presents the first detailed description of branched‐chain and aromatic amino acid catabolism inS. solfataricus.