Significance of measles virus antigen and genome distribution in the CNS in SSPE for mechanisms of viral spread and demyelination
Significance of measles virus antigen and genome distribution in the CNS in SSPE for mechanisms of viral spread and demyelination
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DOI：
10.1097/00005072-199604000-00010
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发表时间：
1996-04-01
影响因子：
3.2
通讯作者：
McConnell, R
中科院分区：
文献类型：
--
作者：
Allen, IV;McQuaid, S;McConnell, R
应用免疫细胞化学和原位杂交技术对10例亚急性硬化性全脑炎（SSPE）患者中枢神经系统（CNS）内麻疹病毒（MV）抗原和基因组RNA的分布进行了研究。在以前的报告中，神经元和少突胶质细胞被发现是最常见的感染细胞。经证实，MV感染的神经元过程主要是树突状，但也有一些证据表明偶尔轴突参与，电子显微镜证实的发现。此外，MV基因组RNA中检测到神经元的过程中，在某些情况下，在没有明显的MV抗原。髓鞘破坏与少突胶质细胞感染之间的关系提示，脱髓鞘可能是病毒感染的唯一结果。研究了病毒分布和形式与疾病临床持续时间之间的可能相关性。在大多数病程短（< 6个月）的病例中，病毒抗原和基因组在大脑皮层中同样丰富。然而，在其中两例病例中，在脊髓中检测到病毒RNA而不是抗原。在长期病例（> 36个月）中，病毒RNA在CNS检查的所有区域都很丰富，通常在缺乏可证实的抗原的情况下。这些发现可能表明病毒在头-尾方向传播，可能通过跨神经元传播。
The distribution of measles virus (MV) antigen and genomic RNA in the central nervous system (CNS) in 10 cases of subacute sclerosing panencephalitis (SSPE) was determined using optimized immunocytochemistry and in situ hybridization techniques. As in previous reports neurons and oligodendrocytes were found to be the most frequently infected cells. It was confirmed that MV infection in neuronal processes was predominantly dendritic but there was also some evidence suggestive of occasional axonal involvement, a finding confirmed by electron microscopy. In addition MV genomic RNA was detected in neuronal processes, in some cases in the absence of demonstrable MV antigen. The relationship between myelin destruction and oligodendrocytic infection suggested that the demyelination may be solely the result of virus infection. A possible correlation between viral distribution and form and the clinical duration of disease was examined. Viral antigen and genome were equally abundant in the cerebral cortex in most short duration cases (< 6 months). However, in two of these cases viral RNA but not antigen was detected in the spinal cord. In long duration cases (> 36 months) viral RNA was abundant in all areas of the CNS examined, frequently in the absence of demonstrable antigen. These findings may suggest viral spread in a cephalo-caudal direction, probably by transneuronal spread.