Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost
Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost
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DOI：
10.1016/j.soilbio.2006.03.019
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发表时间：
2006-09-01
影响因子：
9.7
通讯作者：
Pletschke, Brett I.
中科院分区：
文献类型：
--
作者：
Mayende, Lungisa;Wilhelmi, Brendan S.;Pletschke, Brett I.
在堆肥中，有机物质在通常高于50摄氏度的温度下被纤维素酶和木质素分解酶降解。本研究从堆肥系统中分离嗜热微生物，然后筛选纤维素酶和多酚氧化酶活性。纤维素酶和多酚氧化酶的最适温度分别确定为70 ℃和40 ℃。最大纤维素酶活性测定为1.333 mg葡萄糖释放ml（-1）min（-1）。多酚氧化酶的最大活性为5.111 nmol苯酚ml（-1）min（-1）。发现纤维素酶在1小时内是稳定的。经16 S rDNA序列分析，初步鉴定为芽孢杆菌属（Bacillus）。(c)2006爱思唯尔有限公司保留所有权利。
In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 degrees C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 degrees C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml(-1) min(-1). Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml(-1) min(-1). Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis. (c) 2006 Elsevier Ltd. All rights reserved.