Differences in the susceptibility of human blood cell lines to vaccinia virus.
Differences in the susceptibility of human blood cell lines to vaccinia virus.
复制标题
人类血细胞系对痘苗病毒的敏感性差异。
DOI：
10.1159/000150024
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发表时间：
1988
期刊：
影响因子：
4.6
通讯作者：
Friend,C
中科院分区：
文献类型：
--
作者：
Pogo,BG;Lai,AC;Holland,JG;Friend,C
研究了牛痘病毒在人造血细胞系中的复制，以确定细胞类型和分化阶段是否会影响感染的结果。淋巴细胞NB104细胞和髓性白血病K562细胞可被牛痘有效感染。NB104细胞容易裂解，而K562细胞持续感染。早幼粒细胞白血病hl60细胞感染导致感染流产，病毒未被包被，不复制，保持潜伏，可通过与受纳细胞共培养而获救。在hl60细胞培养物中加入磷酸酯后，病毒复制恢复。这些结果表明，尽管牛痘病毒的复制几乎与宿主特异性功能无关，但感染的结果可能取决于细胞类型及其分化阶段。
The replication of vaccinia virus in human hematopoietic cell lines was studied, to ascertain whether the cell type and the stage of differentiation can influence the outcome of the infection. Lymphocytic NB104 cells and myelogenous leukemic K562 cells can be productively infected with vaccinia. Whereas NB104 cells were readily lysed, cells from the K562 line stayed persistently infected. Infection of promyelocytic leukemia HL60cells results in an abortive infection in which the virus is uncoated, does not replicate, remains latent and can be rescued by cocultivation with permissive cells. Upon addition of phorbol esters to HL60cell cultures, viral replication resumed. These results suggests that, although vaccinia virus replication is almost independent of host-specific functions, the outcome of the infection may depend on the cell type and its stage of differentiation.