Comparative aspects of the metabolism and excretion of cortisol in three individual nonhuman primates
Comparative aspects of the metabolism and excretion of cortisol in three individual nonhuman primates
复制标题
DOI：
10.1006/gcen.1999.7431
复制
发表时间：
2000-03-01
影响因子：
2.7
通讯作者：
Heistermann, M
中科院分区：
文献类型：
--
作者：
Bahr, NI;Palme, R;Heistermann, M
一项放射性代谢研究为三种非人类灵长类动物（普通狨猴（Callithrix jacchus）、长尾猕猴（Macaca fascicularis）和黑猩猩（Pan troglodytes））分泌[H-3]皮质醇代谢物的时间过程、途径和特征提供了第一个比较数据。将低剂量（40-100 μ Ci）的h -3标记的皮质醇静脉注射给每个物种的一只成年雄性，并在注射后5天内收集排泄物。放射性物质主要通过尿液排出（bbb80 %）。三种动物在注射后5.5 h内尿液中的放射性峰值均恢复，而在注射后26 h内粪便中的放射性峰值均恢复。在这三个物种中，尿液代谢物主要以偶联物的形式排出（61-87%），而粪便中偶联代谢物的比例为50%或更低。通过反相高效液相色谱（HPLC）测定尿液和粪便[H-3]皮质醇代谢物的数量和相对丰度，并通过使用已建立的皮质醇、皮质酮和11-氧etiocholanolone酶免疫测定法（EIA）筛选HPLC组分来评估放射性峰的免疫反应性，EIA是一种用于测量11,17-二氧雄甾烯的群体特异性测定法。尿液和粪便提取物的高效液相色谱分离显示出多个放射性峰，其中一些是所有三种物种共同的，然而这些峰的相对比例在物种之间以及尿液和粪便之间存在很大差异，高效液相色谱表明天然皮质醇是狨猴尿液排泄产物的主要产物，而猕猴和黑猩猩的尿液中存在相对较少的量。相比之下，在狨猴的粪便中，皮质醇的含量很低，猕猴和黑猩猩的粪便中几乎没有皮质醇。在这三个物种中，其中一个主要的放射性峰显示出与11-氧乙酯胆甾醇酮相当的滞留时间，并且在11-氧乙酯胆甾醇酮EIA中具有较高的免疫反应性。因此，尿和/或粪便免疫反应性11,17-二氧雄甾的测定与无创评估有关；旧大陆猴、新大陆猴和类人猿肾上腺功能的变化。(C) 2000年学术出版社。
A radiometabolism study is described to provide the first comparative data on the time course, route, and characteristics of excreted [H-3]cortisol metabolites in three nonhuman primates: the common marmoset (Callithrix jacchus), the long-tailed macaque (Macaca fascicularis), and the chimpanzee (Pan troglodytes). A low dose (40-100 mu Ci) of H-3-labeled cortisol was administered intravenously to one adult male of each species and the excreta collected over a 5-day period postinjection. The major proportion of radioactivity was excreted in the urine (>80%). Peak radioactivity in urine was recovered within 5.5 h following injection in all three species, while in the feces peak levels of radioactivity were recovered within 26 h postinjection. In all three species, urinary metabolites were primarily excreted as conjugates (61-87%), whereas the percentage of conjugated metabolites in feces was 50% or less. The number and relative abundance of urinary and fecal [H-3]cortisol metabolites were determined by reverse-phase highperformance liquid chromatography (HPLC) and immunoreactivity of the radioactivity peaks was assessed by screening HPLC fractions with established cortisol, corticosterone, and 11-oxoetiocholanolone enzyme immunoassays (EIA), the latter being a group-specific assay for measuring 11,17-dioxoandrostanes. HPLC separation of urinary and fecal extracts revealed multiple peaks of radioactivity, several of which were common to all three species, The relative proportion of these peaks, however, differed considerably among species and between urine and feces, HPLC indicated that native cortisol was a major urinary excretory product in the marmoset, while comparatively small amounts were present in the urine of the macaque and chimpanzee. In contrast, in feces, cortisol was only detected in low amounts in the marmoset and was virtually absent in the macaque and chimpanzee. In all three species, one of the major radioactivity peaks showed a retention time comparable to 11-oxoetiocholanolone and high immunoreactivity in the 11-oxoetiocholanolone EIA. The measurement of urinary- and/or fecal-immunoreactive 11,17-dioxoandrostanes is therefore implicated for noninvasive assess; ment of adrenal function in Old World monkeys, New World monkeys, and great apes. (C) 2000 Academic Press.