Changes in P2Y2 receptor localization on adrenaline- and noradrenaline-containing chromaffin cells in the rat adrenal gland during development and aging
Changes in P2Y2 receptor localization on adrenaline- and noradrenaline-containing chromaffin cells in the rat adrenal gland during development and aging
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DOI：
10.1016/j.ijdevneu.2005.07.004
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发表时间：
2005-11-01
影响因子：
1.8
通讯作者：
Burnstock, G
中科院分区：
文献类型：
--
作者：
Afework, M;Burnstock, G
应用免疫组织化学方法，观察了胚胎E16 ~ 22月龄大鼠肾上腺中P2 Y（2）受体的分布及其随年龄的变化。P2 Y（2）受体的免疫反应性存在于嗜铬细胞和神经纤维在所有年龄检查。用抗苯乙醇胺-N-甲基转移酶（标记肾上腺素产生的嗜铬细胞）的抗体进行双标记，发现在胚胎E16天时，只有少数P2 Y（2）免疫反应阳性细胞产生肾上腺素，绝大多数是含去甲肾上腺素的细胞。然而，在P2 Y（2）受体标记的嗜铬细胞中，含肾上腺素的细胞的免疫反应性随着年龄的增加而增加，在出生后1周时，几乎所有的嗜铬细胞都对P2 Y（2）和苯基乙醇胺-N-甲基转移酶呈阳性，而含去甲肾上腺素的细胞很少。在2周时，P2 Y（2）免疫反应性嗜铬细胞急剧下降，这在成年大鼠中得以维持，含去甲肾上腺素的细胞占主导地位。在衰老大鼠肾上腺中，P2 Y（2）受体免疫反应性定位于肾上腺素和去甲肾上腺素产生细胞的亚群中。在成年和老年大鼠的肾上腺中，也可见被P2 Y（2）受体抗体阳性标记的内源性神经元。P2 Y（2）免疫反应阳性神经纤维在出生后的大鼠肾小球肾上腺皮质细胞周围形成丛，而在成年或衰老大鼠中则不形成丛，提示ATP可能通过P2 Y（2）受体影响大鼠肾上腺发育和衰老过程中嗜铬细胞的表型表达。然而，在早期发育过程中，当嗜铬细胞活跃分裂时，以及在衰老过程中，当肾上腺髓质细胞显示增生性病变时，通过P2 Y（2）受体起作用的ATP可能参与其他生理活动，例如与肾上腺素或去甲肾上腺素表型相关的嗜铬细胞的增殖和/或分化。(c)2005年ISDN。由爱思唯尔有限公司出版。保留所有权利。
Using immunohistochemistry, the occurrence and age-related changes of the P2Y(2) receptor was investigated in the adrenal gland of rat at different ages, ranging from embryonic day E16 to 22 months. Immunoreactivity for the P2Y(2) receptor was present in chromaffin cells and nerve fibres at all ages examined. Double labeling with the antibody against phenyl ethanolamine-N-methyltransferase, which marks adrenaline-producing chromaffin cells, revealed that only a few of the P2Y(2)-immunoreactive cells were adrenaline producing at embryonic day E16, the vast majority being noradrenaline-containing cells. However, immunoreactivity for adrenaline-containing cells in the P2Y(2) receptor-labeled chromaffin cells increased with increasing age and at 1 week post-natal almost all chromaffin cells were positive for both P2Y(2) and phenyl ethanolamine-N-methyltransferase, while noradrenaline-containing cells were minimal. At 2 weeks, there was a dramatic drop in P2Y(2)-immunoreactive chromaffin cells and this was maintained in adult rats, noradrenaline-containing cells dominating. In the aging rat adrenals, P2Y(2) receptor-immunoreactivity was localized in subpopulations of both adrenaline and noradrenaline-producing cells. Intrinsic neurones were also visible that were positively labeled with the P2Y(2) receptor antibody in the adrenals of both adult and aging rats. P2Y(2)-immunoreactive nerve fibres formed a plexus around the adrenal cortical cells of zona glomerulosa in the post-natal, but not in adult or aging rats.In conclusion, this study suggests that ATP, acting through P2Y(2) receptors, may influence the phenotypic expression of chromaffin cells during the development and aging of the rat adrenal gland. However, during early development, when the chromaffin cells are actively dividing and during aging, when the adrenal medullary cells are known to show hyperplastic lesions, ATP acting through P2Y(2) receptors may be involved in other physiological activities, such as proliferation and/or differentiation of the chromaffin cells associated with their adrenaline or noradrenaline phenotype. (c) 2005 ISDN. Published by Elsevier Ltd. All rights reserved.