FIBRONECTIN AND FIBRIN PROVIDE A PROVISIONAL MATRIX FOR EPIDERMAL-CELL MIGRATION DURING WOUND REEPITHELIALIZATION
FIBRONECTIN AND FIBRIN PROVIDE A PROVISIONAL MATRIX FOR EPIDERMAL-CELL MIGRATION DURING WOUND REEPITHELIALIZATION
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DOI：
10.1111/1523-1747.ep12500075
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发表时间：
1982-01-01
影响因子：
6.5
通讯作者：
COLVIN, RB
中科院分区：
文献类型：
--
作者：
CLARK, RAF;LANIGAN, JM;COLVIN, RB
在创伤愈合中，调节再生表皮附着和定向迁移的因素知之甚少。在豚鼠4-mm皮肤伤口的研究中，暴露1-28天，进行活组织检查并处理1-μ m切片和免疫荧光，表皮在含有纤维蛋白和纤连蛋白的不规则增厚的临时基质上迁移。临时基质缺乏正常基底膜的2种主要成分，层粘连蛋白和IV型胶原，其可以在体外介导坚韧的上皮与塑料的附着，并可能限制体内表皮细胞的迁移。在创伤后7-9天完成伤口再上皮化后，基底膜区失去其增厚的外观，纤维连接蛋白和纤维蛋白原消失，IV型胶原和层粘连蛋白重新出现。虽然这些发现并不能证明表皮细胞在再上皮化过程中的迁移需要纤维蛋白和纤连蛋白基质，但它们证明了表皮细胞在体内伤口修复过程中确实在这样的基质上移动。
Factors regulating the attachment and directional migration of a regenerating epidermis in wound healing are poorly understood. In studies of guinea pig 4-mm skin wounds, left uncovered for 1-28 days, biopsied and processed for 1-.mu.m section and immunofluorescence, the epidermis migrated over an irregularly thickened provisional matrix containing fibrin and fibronectin. The provisional matrix lacked 2 major components of normal basement membrane, laminin and type IV collagen, which can mediate tenacious epithelial attachment to plastic in vitro and may limit epidermal cell migration in vivo. Upon completion of wound reepithelialization at 7-9 days after wounding, the basement membrane zone lost its thickened appearance, fibronectin and fibrinogen disappeared, and type IV collagen and laminin reappeared. Although these findings do not prove that epidermal cell migration during reepithelialization requires a fibrin and fibronectin matrix, they demonstrate that epidermal cells do move over such a substratum during in vivo wound repair.