Laparoscopic intra-uterine insemination of fallow deer with frozen-thawed or fresh semen after synchronisation with CIDR devices.
Laparoscopic intra-uterine insemination of fallow deer with frozen-thawed or fresh semen after synchronisation with CIDR devices.
复制标题
DOI：
10.1080/00480169.1992.35689
复制
发表时间：
1992-03
影响因子：
1.4
通讯作者：
G. Asher;C. Morrow;H. Jabbour;R. Mulley;F. Veldhuizen;M. Langridge
中科院分区：
文献类型：
--
作者：
G. Asher;C. Morrow;H. Jabbour;R. Mulley;F. Veldhuizen;M. Langridge
本研究调查了使用冻融或新鲜精液对养殖小鹿（Dama dama）进行固定时间腹腔镜子宫内授精的效果。在冷冻解冻精液试验中，新西兰五个农场总共使用了 547 只成熟的非哺乳母鹿。对于发情同步和人工授精，每个猪场至少 30% 的个体采用标准控制方案，包括在阴道内插入单个 CIDR G 型装置 14 天，在撤回 CIDR 装置后 65 小时沉积 50 x 10(6) 冻融精子，以及从插入 CIDR 装置到授精后 10 天持续存在输精管切除的雄鹿。对于每个农场的剩余剂量，该协议的各个方面都发生了变化，包括 60 或 70 小时的授精、没有输精管结扎的雄鹿、用 25 x 10(6) 或 10 x 10(6) 精子授精、与 CIDR S 型设备同步以及与前列腺素同步。根据受精后 45 天的直肠超声检查，所有治疗的受孕率为 67% (n=547)。根据农场间调整计算得出的校正受孕率 (+/-.e.)，对照方案为 67+/- 3%，60 小时和 70 小时授精分别为 67+/- 9% 和 73 +/- 8%，无雄鹿授精为 61 +/- 9%，25 x 10(6) 和 10 授精为 80 +/- 8% 和 74 +/- 9%分别为 x 10(6) 个精子，CIDR S 型设备同步为 62 +/- 10%，前列腺素同步为 49 +/- 10%。尽管存在明显差异，但没有一种治疗导致调整后的受孕率与对照方案有显着差异（P>0.01）。在使用新鲜精液的试验中，美国的 216 只母鹿在 CIDR 装置撤回后 69-71 小时内进行了授精，使用来自新西兰的冷冻精液（n=158；每次授精 25 x 10(6) 精子）或新鲜精液（n=58；每次授精 7.5 x10(6) 至 20 x 10(6) 精子）收集的少于10个小时前。总体受孕率分别为77%和81%，精液类型（冷冻与新鲜）或每次授精的新鲜精子数量之间没有显着差异（P>0.01）。新西兰的另外 102 只母鹿也同样接受了来自 3/4 美索不达米亚雄鹿的新鲜精液。撤回 CIDR 装置后 69-71 小时，每次授精输送 10 x 10(6) (n=35)、5 x 10(6) (n=32) 或 2.5 x 10(6) (n=35) 精子的剂量。受孕率分别为77%、66%和51%，反映了剂量效应（P<0.05）。然而，无论精液剂量如何，该组中有 1/4 美索不达米亚人 (n=19) 表现出较高的受孕率（总体为 95%），这可能表明精液/受体基因型相互作用。结论是，在去除 CIDR 装置后，以固定时间间隔用冻融或新鲜精液对小鹿进行腹腔镜子宫内授精，在一系列农场管理选项和精液剂量下，可以得到可接受的受孕率。
This study investigated the efficacy of fixed-time laparoscopic intra-uterine insemination of farmed fallow deer (Dama dama) with frozen-thawed or fresh semen. In the trials with frozen-thawed semen, a total of 547 mature non-lactating does across five New Zealand farms were used. For oestrous synchronisation and artificial insemination, a standard control regimen was applied to at least 30% of the does on each farm, involving the insertion of single CIDR type-G devices intravaginally for 14 days, deposition of 50 x 10(6) frozen-thawed spermatozoa at 65 hours after withdrawal of the CIDR device and the continuous presence of vasectomised bucks from the insertion of the CIDR device until 10 days after insemination. Various aspects of this protocol were changed for the remaining does on each farm, including inseminations at 60 or 70 hours, the absence of vasectomised bucks, insemination with 25 x 10(6) or 10 x 10(6) spermatozoa, synchronisation with CIDR type-S devices and synchronisation with prostaglandin. The conception rate, based on rectal ultrasonography at 45 days after insemination, was 67% across all treatments (n=547). Corrected conception rates (+/-s.e.), calculated following between-farm adjustments, were 67+/- 3% for the control regimen, 67+/- 9% and 73 +/- 8% for inseminations at 60 and 70 hours respectively, 61 +/- 9% for absence of bucks, 80 +/- 8% and 74 +/- 9% for inseminations with 25 x 10(6) and 10 x 10(6) spermatozoa respectively, 62 +/- 10% for CIDR type-S device synchronisation, and 49 +/- 10% for prostaglandin synchronisation. Despite apparent differences, none of the treatments resulted in adjusted conception rates that were significantly different from the control regimen (P>0.01). In the trials with fresh semen, 216 does in the USA were inseminated at 69-71 hours after withdrawal of the CIDR device using either cryopreserved semen from New Zealand (n=158; 25 x 10(6) spermatozoa per inseminate) or fresh semen (n=58; 7.5 x10(6) to 20 x 10(6) spermatozoa per inseminate) collected less than 10 hours earlier. The overall conception rates were 77% and 81% respectively, with no significant differences between semen type (frozen v. fresh) or fresh spermatozoa number per inseminate (P>0.01). A further 102 does in New Zealand similarly received fresh semen from 3/4 Mesopotamian buck. Doses of 10 x 10(6) (n=35), 5 x 10(6) (n=32) or 2.5 x 10(6) (n=35) spermatozoa per inseminate were delivered at 69-71 hours after withdrawal of the CIDR device. The conception rates were 77%, 66% and 51% respectively, reflecting a dose effect (P<0.05). However, 1/4 Mesopotamian does in the group (n=19) exhibited higher conception rates (95% overall) irrespective of semen dose, possibly indicating a semen/recipient genotype interaction. It is concluded that laparoscopic intra-uterine insemination of fallow deer with frozen-thawed or fresh semen at fixed intervals after removal of a CIDR device can give acceptable conception rates under a range of on-farm management options and semen doses.