N-methylation of purines and pyrimidines.
N-methylation of purines and pyrimidines.
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嘌呤和嘧啶的 N-甲基化。
DOI：
10.1016/0003-2697(75)90492-3
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发表时间：
1975
影响因子：
2.9
通讯作者：
P. Klein
中科院分区：
文献类型：
--
作者：
W. F. Bryant;P. Klein
建立了一种简便、快速的嘌呤和嘧啶N-甲基化方法。当嘌呤或嘧啶溶解在DMSO中并用甲醇钠和碘甲烷处理时，发生定量转化为N-全甲基化产物。胸腺嘧啶、腺嘌呤、6-甲氨基嘌呤、黄嘌呤和尿酸都是用这种方法衍生的。在胸腺嘧啶、黄嘌呤和尿酸的情况下，没有观察到O-甲基化的证据。给出了甲基化产物的化学电离质谱和电子轰击质谱。胸腺嘧啶甲基化产物的分光光度分析表明定量转化为1，3-二甲基胸腺嘧啶。
A rapid, convenient method for the N-methylation of purines and pyrimidines has been developed. When the purine or pyrimidine is dissolved in DMSO and treated with sodium methoxide and methyl iodide, quantitative conversion to the N-permethylated product occurs. Thymine, adenine, 6-methylaminopurine, xanthine, and uric acid have been derivatized in this manner. In the case of thymine, xanthine, and uric acid no evidence for O-methylation has been observed. The chemical ionization and electron impact mass spectra of the methylated products are presented. Spectrophotometric analysis of the product from the methylation of thymine indicates quantitative conversation to 1,3-dimethylthymine.