Identification and gene cloning of a male-sterile mutant Oswbc11 in rice
Identification and gene cloning of a male-sterile mutant Oswbc11 in rice
复制标题
水稻雄性不育突变体Oswbc11的鉴定及基因克隆
DOI：
10.1111/pbr.12689
复制
发表时间：
2019
期刊：
影响因子：
2
通讯作者：
Ma Bojun
中科院分区：
文献类型：
--
作者：
Xuan Changqing;Liu Yaping;Liu Chengjie;Lusuwi Lewis Bongani Tafadzwa;Fu Xiaojin;Chen Xifeng;Ma Bojun
最近，一项新的技术——种子生产技术（SPT）提高了核遗传雄性不育的商业应用，该技术结合了转基因保持系，能够繁殖非转基因核雄性不育系，作为杂交生产中的母本。在这里，我们发现了一个水稻核雄性不育突变体Oswbc11，花粉发育和脂质转运异常。我们精细地将oswbc11基因定位到10号染色体上一个12.5 kb的区域，并找到了一个候选基因，该基因具有碱基替换（C到T）导致过早停止密码子，并通过CRISPR/Cas9技术在功能上进行了证实。geneoswbc11编码三磷酸腺苷结合盒转运蛋白亚家族成员，参与多种分子跨膜的主动转运。此外，除结实率外，oswbc11突变体的农艺性状与野生型对照没有显著差异。上述结果表明，oswbc11基因可作为隐性核雄性不育基因与CRISPR/Cas9和SPT技术结合，在水稻杂交育种中应用。
Commercial application of nuclear genetic male sterility has been improved recently by a novel technique, Seed Production Technology (SPT), which incorporates transgenic maintainer lines capable of propagating non‐transgenic nuclear male‐sterile lines for use as female parents in hybrid production. Here, we identified a rice nuclear male‐sterile mutant,Oswbc11, with abnormal pollen development and lipid transport. We finely mapped theOswbc11gene into a 12.5‐kb region on chromosome 10 and found one candidate gene, which had a base substitution (C to T) resulting in a premature stop codon and was functionally confirmed by CRISPR/Cas9 technology. GeneOsWBC11encodes a sub‐family member of adenosine triphosphate‐binding cassette transporter, which participates in the active transport of a wide range of molecules across membranes. Moreover, the agronomic traits ofOswbc11mutants showed no significant differences compared to the wild‐type control except for the seed setting rate. These results indicated thatOswbc11gene could be used in rice hybrid breeding as a recessive nuclear male‐sterile gene combined with CRISPR/Cas9 and the SPT technology and applied in different rice varieties.