Measuring volume kinetics of human monocytes in response to cryoprotectants using microfluidic technologies
Measuring volume kinetics of human monocytes in response to cryoprotectants using microfluidic technologies
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DOI：
10.1063/1.5096199
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发表时间：
2019-06
影响因子：
4
通讯作者：
Rekha Raju;Hannes Höhn;C. Karnutsch;K. Khoshmanesh;G. Bryant
中科院分区：
文献类型：
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作者：
Rekha Raju;Hannes Höhn;C. Karnutsch;K. Khoshmanesh;G. Bryant
冷冻保存是保存生物细胞和组织的常用策略。虽然常规平台如细胞培养孔板系统能够测量细胞对各种冷冻保护剂的响应，但与细胞的捕获和成像相关的缺点限制了此类系统的实用性。微流体技术有助于使用低样本量进行细胞捕获、化学刺激和成像。在这里，我们利用微流体技术的流体动力学捕获单个人单核细胞和研究细胞体积动力学响应于冷冻保护剂在真实的时间。我们的方法有助于进行多步细胞测定，特别是用于研究单个细胞的渗透反应和确定细胞膜对冷冻保护剂的渗透性。冷冻保存是保存生物细胞和组织的常用策略。虽然常规平台如细胞培养孔板系统能够测量细胞对各种冷冻保护剂的响应，但与细胞的捕获和成像相关的缺点限制了此类系统的实用性。微流体技术有助于使用低样本量进行细胞捕获、化学刺激和成像。在这里，我们利用微流体技术的流体动力学捕获单个人单核细胞和研究细胞体积动力学响应于冷冻保护剂在真实的时间。我们的方法有利于进行多步细胞测定，特别是用于研究单个细胞的渗透反应和确定细胞膜对冷冻保护剂的渗透性。
Cryopreservation is a common strategy for the preservation of biological cells and tissues. While conventional platforms such as cell culture well plate systems enable measuring cell responses to various cryoprotectants, the drawbacks associated with capturing and imaging of cells limit the utility of such systems. Microfluidic technologies facilitate the capturing, chemical stimulation, and imaging of cells using low sample volumes. Here, we utilized microfluidic technologies for the hydrodynamic capturing of single human monocytes and studying the cell volume kinetics in response to a cryoprotectant in real time. Our approach facilitates conducting multistep cellular assays, especially for studying individual cell osmotic response and determining cell membrane permeability to cryoprotectants.Cryopreservation is a common strategy for the preservation of biological cells and tissues. While conventional platforms such as cell culture well plate systems enable measuring cell responses to various cryoprotectants, the drawbacks associated with capturing and imaging of cells limit the utility of such systems. Microfluidic technologies facilitate the capturing, chemical stimulation, and imaging of cells using low sample volumes. Here, we utilized microfluidic technologies for the hydrodynamic capturing of single human monocytes and studying the cell volume kinetics in response to a cryoprotectant in real time. Our approach facilitates conducting multistep cellular assays, especially for studying individual cell osmotic response and determining cell membrane permeability to cryoprotectants.