The isolation and in vitro expansion of hepatic Sca-1 progenitor cells
The isolation and in vitro expansion of hepatic Sca-1 progenitor cells
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DOI：
10.1016/j.bbrc.2009.02.079
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发表时间：
2009-04-17
影响因子：
3.1
通讯作者：
Forbes, Stuart J.
中科院分区：
文献类型：
--
作者：
Clayton, Elizabeth;Forbes, Stuart J.
当肝细胞增殖受到抑制时，肝损伤期间肝内的肝祖细胞群会扩张。这些细胞可以通过密度梯度离心纯化并培养。仅按大小区分，该细胞群含有造血细胞、上皮细胞和内皮细胞谱系的小细胞，并且被认为含有肝干细胞。尽管有一些证据表明组织Sca1(+) CD45(-)细胞表现出祖细胞特征，但肝脏干细胞的身份仍然未知。我们鉴定了肝损伤后肝内和胆囊中的 Sca1(+) 细胞，并体外扩增了 Sca1 细胞作为异质细胞培养物的一部分或作为纯化群体。我们发现当铺在层粘连蛋白或 I 型胶原蛋白上时，Sca-1 细胞的增殖存在显着差异，而异源细胞群的增殖不受细胞外基质的影响，表明在长期肝祖细胞培养中用层粘连蛋白基质或层粘连蛋白产生细胞培养 Sca1(+) 细胞的必要性。 (C) 2009 Elsevier Inc. 保留所有权利。
The intra-hepatic population of liver progenitor cells expands during liver injury when hepatocyte proliferation is inhibited. These cells can be purified by density gradient centrifugation and cultured. Separated by size only this Population contains small cells of hematopoietic, epithelial and endothelial lineages and is thought to contain liver stem cells. The identity of liver stem cells remains unknown although there is some evidence that tissue Sca1(+) CD45(-) cells display progenitor cell characteristics. We identified both intra-hepatic and gall bladder Sca1(+) cells following liver injury and expanded ex vivo Sca1 cells as part of heterogenous Cell culture or as a Purified population. We found significant difference between the proliferation of Sca-1 cells when plated on laminin or Collagen I while proliferation of heterogenous Population was not affected by the extracellular matrix indicating the necessity for Culture of Sca1(+) cells with laminin matrix or laminin producing cells in long term liver progenitor cell cultures. (C) 2009 Elsevier Inc. All rights reserved.