On-Off-On Fluorescent Chemosensors Based on N/P-Codoped Carbon Dots for Detection of Microcystin-LR
On-Off-On Fluorescent Chemosensors Based on N/P-Codoped Carbon Dots for Detection of Microcystin-LR
复制标题
On-Off-On 基于 N/P 共掺杂碳点的荧光化学传感器用于检测微囊藻毒素-LR
DOI：
10.1021/acsanm.1c00921
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发表时间：
2021
影响因子：
5.9
通讯作者：
Chen Lingxin
中科院分区：
文献类型：
--
作者：
Wang Xiaoyan;Yu Jialuo;Ji Wan;Arabi Maryam;Fu Longwen;Li Bowei;Chen Lingxin
微囊藻毒素-LR（MC-LR）作为一种非荧光物种的传感仍然是具有挑战性的，因为它对荧光纳米材料的发射具有可忽略的影响。在此，一个定义明确的“开-关-开”的间接化学传感策略是通过氮/磷共掺杂的碳量子点（N/P CD）开发的高灵敏度和选择性的MC-LR测定。以5′-三磷酸腺苷（ATP）为原料，采用水热碳化法一步合成了纳米级N/P环糊精。由于Fe ~（3+）与N/P环糊精的磷酸基团形成了Fe-O-P键，使N/P环糊精的荧光强度在Fe ~（3+）存在下发生猝灭。N/P CD-Fe ~（3+）体系对MC-LR反应敏感，且由于MC-LR空腔与Fe ~（3+）竞争性配位，其荧光强度迅速恢复。在优化条件下，该传感器对MC-LR的线性范围为0.05 ~ 3 μg/L，检出限为17.1 ng/L。将该传感器应用于真实的水样中MC-LR的准确检测，回收率在95.0- 109.3%之间，相对标准偏差在1.8- 4.7%之间。利用荧光化学传感器的优点，如操作简单、生产成本低、响应时间快、选择性好、灵敏度高等，该传感平台可用于复杂环境水样中MC-LR的检测。
The sensing of microcystin-LR (MC-LR) as a nonfluorescent species is still challenging since it has a negligible influence on the emission of fluorescent nanomaterials. Herein, a well-defined “on–off–on” indirect chemosensing strategy is developed by means of nitrogen/phosphorus-codoped carbon dots (N/P CDs) for highly sensitive and selective MC-LR determination. The nanoscale N/P CDs were conveniently synthesized by one-step hydrothermal carbonization of adenosine 5′-triphosphate (ATP) in aqueous media. The fluorescence intensity of the N/P CDs was quenched in the presence of Fe3+due to the formation of Fe–O–P bonds between Fe3+and the phosphate groups of the N/P CDs. The resulting N/P CD-Fe3+system was sensitive to MC-LR, and its fluorescence intensity recovered dramatically and quickly since the MC-LR cavity competitively coordinated with Fe3+. Under optimized conditions, the proposed chemosensor exhibited a wide linear response for MC-LR in the range of 0.05–3 μg/L, with a detection limit down to 17.1 ng/L. Moreover, the chemosensor was successfully applied to accurately detect MC-LR in real water samples and achieved high recoveries in the range of 95.0–109.3%, with relative standard deviations within 1.8–4.7%. By relying on the benefits of fluorescent chemosensors, such as simple operation, low production cost, fast response time, selectivity, and sensitivity, the proposed sensing platform possesses promising applicability for MC-LR determination in complex environmental water samples.