Red emissive AIE nanodots with high two-photon absorption efficiency at 1040 nm for deep-tissue in vivo imaging
Red emissive AIE nanodots with high two-photon absorption efficiency at 1040 nm for deep-tissue in vivo imaging
复制标题
红色发射 AIE 纳米点在 1040 nm 处具有高双光子吸收效率，用于深部组织体内成像
DOI：
10.1364/boe.6.003783
复制
发表时间：
2015-10-01
影响因子：
3.4
通讯作者：
Qian, Jun
中科院分区：
文献类型：
--
作者：
Wang, Yalun;Hu, Rongrong;Qian, Jun
在活体光学生物成像中，对深层组织穿透的要求很高。合成了一种具有聚集诱导发光（AIE）性质的红色荧光团BT。然后用两亲聚合物封装BT分子以形成纳米点，并且从每个BT纳米点观察到在1040 nm处的2.9 x 10（6）GM的大的双光子吸收（2PA）截面，其远大于在770至860 nm波长处的那些。此外，根据Monte Carlo模拟，发现1040 nm光在生物组织中具有比800 nm光更好的穿透和聚焦能力。研究了BT纳米点的毒性和组织分布，发现它们具有良好的生物相容性。BT纳米点，然后用于在小鼠耳和脑的体内成像，并获得了700 μ m的成像深度与飞秒（fs）激发1040 nm。在1040 nm处具有高2PA效率的红色发射AIE纳米点将在未来用于深层组织功能生物成像。(C)2015美国光学学会
Deep-tissue penetration is highly required in in vivo optical bioimaging. We synthesized a type of red emissive fluorophore BT with aggregation-induced emission (AIE) property. BT molecules were then encapsulated with amphiphilic polymers to form nanodots, and a large twophoton absorption (2PA) cross-section of 2.9 x 10(6) GM at 1040 nm was observed from each BT nanodot, which was much larger than those at the wavelengths of 770 to 860 nm. In addition, 1040 nm light was found to have better penetration and focusing capability than 800 nm light in biological tissue, according to the Monte Carlo simulation. The toxicity and tissue distribution of BT nanodots were studied, and they were found to have good biocompatibility. BT nanodots were then utilized for in vivo imaging of mouse ear and brain, and an imaging depth of 700 mu m was obtained with the femtosecond (fs) excitation of 1040 nm. The red emissive AIE nanodots with high 2PA efficiency at 1040 nm would be useful for deep-tissue functional bioimaging in the future. (C) 2015 Optical Society of America