CALCIUM CURRENTS IN SINGLE SA NODAL CELLS OF THE RABBIT HEART STUDIED WITH ACTION-POTENTIAL CLAMP
CALCIUM CURRENTS IN SINGLE SA NODAL CELLS OF THE RABBIT HEART STUDIED WITH ACTION-POTENTIAL CLAMP
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DOI：
10.1007/bf00581808
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发表时间：
1989-04-01
影响因子：
4.5
通讯作者：
TRAUTWEIN, W
中科院分区：
文献类型：
--
作者：
DOERR, T;DENGER, R;TRAUTWEIN, W
通过一种称为“动作电位钳”(APC) 的新方法，我们在单个 SA 节点细胞中研究了瞬时和持久钙电流（L 型和 T 型）对动作电位的贡献。计算机记录动作电位，随后在电压钳下将代表性循环重复重放至同一细胞。 L 型钙电流 (D600) 或 T 型钙电流（镍）的阻断揭示了这些电流对动作电位的定量和时间相关贡献，因为阻断的电流由钳位放大器补偿。
With a new method called "Action Potential-Clamp" (APC) we studied in single SA nodal cells the contribution of both transient and long lasting calcium currents (L-type and T-type) to the action potential. Action potentials were recorded by a computer and a representative cycle was subsequently repetitively replayed to the same cell under voltage clamp. Blockade of the L-type calcium current (D600) or T-type calcium current (nickel) revealed the quantitative and time related contributions of these currents to the action potential, since the blocked current is compensated by the clamp amplifier.