Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration
Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration
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DOI：
10.1016/j.actbio.2011.02.036
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发表时间：
2011-06-01
影响因子：
9.7
通讯作者：
Cooper-White, Justin John
中科院分区：
文献类型：
--
作者：
Vaquette, Cedryck;Cooper-White, Justin John
本研究研究了使用图案化收集器来增加电纺支架的孔径以增强细胞浸润。通过扫描电子显微镜研究了图案支架的形态，结果表明电纺纤维准确地模仿了收集器图案。与传统静电纺丝相比，我们观察到孔径和孔径分布有所扩大。机械测试表明，机械性能可以在一定程度上根据图案进行定制，并且图案化支架比标准静电纺丝支架更柔软。当 NIH 3T3 成纤维细胞接种到图案收集器上时，观察到细胞浸润得到改善。细胞能够渗入支架达 250 μm，而标准支架的渗入深度为 30 μm。渗透深度的增加早在播种后 24 小时就发生，并在 7 天内保持恒定。 Crown 版权所有 (C) 2011 由 Elsevier Ltd. 代表 Acta Materialia Inc. 出版。保留所有权利。
This study investigates the use of patterned collectors to increase the pore size of electrospun scaffolds for enhanced cell infiltration. The morphology of the patterned scaffolds was investigated by scanning electron microscopy, which showed that the collector pattern was accurately mimicked by the electrospun fibres. We observed an enlargement in the pore size and in the pore size distribution compared with conventional electrospinning. Mechanical testing revealed that the mechanical properties could be tailored, to some extent, according to the patterning and that the patterned scaffolds were softer than standard electrospun scaffolds. When NIH 3T3 fibroblasts were seeded onto patterned collectors improved cell infiltration was observed. Cells were able to penetrate up to 250 mu m into the scaffolds, compared with 30 for the standard scaffolds. This increase in the depth of infiltration occurred as early as 24 h post-seeding and remained constant over 7 days. Crown Copyright (C) 2011 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. All rights reserved.