Comparison of 5-aminolevulinic acid-encapsulated liposome versus ethosome for skin delivery for photodynamic therapy
Comparison of 5-aminolevulinic acid-encapsulated liposome versus ethosome for skin delivery for photodynamic therapy
复制标题
DOI：
10.1016/j.ijpharm.2008.01.020
复制
发表时间：
2008-05-22
影响因子：
5.8
通讯作者：
Huang, Yaw-Bin
中科院分区：
文献类型：
--
作者：
Fang, Yi-Ping;Tsai, Yi-Hung;Huang, Yaw-Bin
局部光动力疗法(PDT)与5-氨基乙酰丙酸(ALA)是许多非黑色素瘤皮肤癌的替代疗法。然而，这种疗法的主要局限性是ALA通过皮肤角质层的低渗透性。本工作的目的是表征含有丙氨酸的醇体，并与传统脂质体相比，提高原卟啉IX(PpIX)的皮肤产量。结果表明，醇体的平均粒径小于脂质体。此外，丙氨酸在醇体制剂中的包封率为8%-66%，这取决于所添加的表面活性剂。保存32天后，乙醇小体的颗粒尺寸仍在200 nm左右。一项活体动物研究通过共聚焦激光扫描显微镜(CLSM)观察到PpIX在皮肤中的存在。结果表明，乙醇体经皮吸收能力强于脂质体。与对照(水溶液中的丙氨酸)相比，所有制剂在PpIX强度方面都有11-15倍的增强。此外，比色法在照射后的皮肤中没有发现红斑。结果表明，除PE/CH/SS外，乙醇体制剂的增强率在未辐照组和辐照组之间没有显著差异，这可能是由于PDT辐照过程的光漂白效应所致。(C)2008爱思唯尔B.V.保留所有权利。
Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) is an alternative therapy for many non-melanoma skin cancers. The major limitation of this therapy, however, is the low permeability of ALA through the stratum corneum (SC) of the skin. The objective of the present work was to characterize ethosomes containing ALA and to enhance the skin production of protoporphyrin IX (PpIX), compared to traditional liposomes. Results showed that the average particle sizes of the ethosomes were less than those of liposomes. Moreover, the entrapment efficiency of ALA in the ethosome formulations was 8-66% depending on the surfactant added. The particle size of the ethosomes was still approximately < 200 nm after 32 days of storage. An in vivo animal study observed the presence of PpIX in the skin by confocal laser scanning microscopy (CLSM). The results indicated that the penetration ability of ethosomes was greater than that of liposomes. The enhancements of all the formulations were ranging from 11- to 15-fold in contrast to that of control (ALA in an aqueous solution) in terms of PpIX intensity. In addition, colorimetry detected no erythema in the irradiated skin. The results demonstrated that the enhancement ratio of ethosome formulations did not significantly differ between the non-irradiated and irradiated groups except for PE/CH/SS, which may have been due to a photobleaching effect of the PDT-irradiation process. (C) 2008 Elsevier B.V. All rights reserved.