Antibiotic susceptibility of Escherichia coli dnaK and dnaJ mutants.
Antibiotic susceptibility of Escherichia coli dnaK and dnaJ mutants.
复制标题
大肠杆菌 dnaK 和 dnaJ 突变体的抗生素敏感性。
DOI：
10.1089/107662900419429
复制
发表时间：
2000
影响因子：
2.6
通讯作者：
Anna Jóźwik
中科院分区：
文献类型：
--
作者：
K. Wolska;Edyta Bugajska;Dorota Jurkiewicz;M. Kuć;Anna Jóźwik
The role of two chaperone proteins, DnaK and the cooperating factor DnaJ, in Escherichia coli antibiotic susceptibility to three antibiotics (a beta-lactam, chloramphenicol, tetracycline) has been studied. It was found that null dnaJ and dnaKdnaJ mutants are impaired in the functions leading to antibiotic susceptibility. The secretion of beta-lactamase to the periplasmic space is diminished in both mutants, and the additive effect of the two mutations was observed. The activity of chloramphenicol acetyltransferase is also impaired in an additive manner in both mutant strains. Tetracycline uptake is changed only in the double deletion mutant. These defects were observed only during incubation at high temperature (42 degrees C). Efficient complementation of some of these defects by the wild-type alleles introduced on low-copy number plasmid was achieved. Minimal inhibitory concentrations and the titer of the wild-type strains, delta dnaJ and delta dnaKdnaJ mutants treated with ampicillin, chloramphenicol, and tetracycline were also determined. Higher susceptibility of both mutants to chloramphenicol and tetracycline, as compared to their wild-type parent, was observed only after 1 h preincubation of cultures at 42 degrees C. On the contrary, both mutants were less susceptible to ampicillin than their parent strain.
DnaK 和 DnaJ 热休克蛋白参与大肠杆菌中的蛋白质输出。
影响因子：
10.5
作者：
Wild,J;Altman,E;Yura,T;Gross,CA
通讯作者：
Gross,CA
影响因子：
2.9
作者：
BROWN, RE;JARVIS, KL;HYLAND, KJ
通讯作者：
HYLAND, KJ
大肠杆菌 SecB 蛋白与体内输出的蛋白前体相关。
DOI：
10.1073/pnas.86.14.5320
发表时间：
1989
影响因子：
11.1
作者：
Kumamoto,CA
通讯作者：
Kumamoto,CA