Evaluation of DESS as a storage medium for microbial community analysis
Evaluation of DESS as a storage medium for microbial community analysis
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DOI：
10.7717/peerj.6414
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发表时间：
2019-02-05
期刊：
影响因子：
2.7
通讯作者：
Klassen, Jonathan L.
中科院分区：
文献类型：
--
作者：
Lee, Kevin M.;Adams, Madison;Klassen, Jonathan L.
微生物生态学研究需要准确代表所研究的微生物群落的采样策略。这些群落通常必须从收集地点运输到实验室，然后储存直至可以进行处理。然而，对于如何在运输和储存过程中最好地保护微生物群落缺乏共识。在这里，我们评估了二甲亚砜乙二胺四乙酸饱和盐 (DESS) 溶液作为微生物生态实验广泛适用的防腐剂。我们将蚂蚁 Trachymyrmex septentrionalis 生长的真菌花园储存在 DESS、15% 甘油和磷酸盐缓冲盐水 (PBS) 中，以测试它们对真菌花园微生物群落的影响。与蚁群之间的差异相比，由于防腐剂类型的差异而导致的微生物群落结构的差异很小。此外，DESS 比 15% 甘油或 PBS 更忠实地保留了定义的模拟群落的结构。 DESS 价格低廉，易于运输，并能有效保存微生物群落结构。因此，我们得出结论，DESS 是一种用于微生物生态学研究的有价值的防腐剂。
Microbial ecology research requires sampling strategies that accurately represent the microbial community under study. These communities must typically be transported from the collection location to the laboratory and then stored until they can be processed. However, there is a lack of consensus on how best to preserve microbial communities during transport and storage. Here, we evaluated dimethyl sulfoxide, ethylenediamine tetraacetic acid, saturated salt (DESS) solution as a broadly applicable preservative for microbial ecology experiments. We stored fungus gardens grown by the ant Trachymyrmex septentrionalis in DESS, 15% glycerol, and phosphate buffered saline (PBS) to test their impact on the fungus garden microbial community. Variation in microbial community structure due to differences in preservative type was minimal when compared to variation between ant colonies. Additionally, DESS preserved the structure of a defined mock community more faithfully than either 15% glycerol or PBS. DESS is inexpensive, easy to transport, and effective in preserving microbial community structure. We therefore conclude that DESS is a valuable preservative for use in microbial ecology research.