AtMYB12-ExpressingTransgenic Tobacco Increases Resistance to Several Phytopathogens and Aphids
AtMYB12-ExpressingTransgenic Tobacco Increases Resistance to Several Phytopathogens and Aphids
复制标题
表达 AtMYB12 的转基因烟草增强了对多种植物病原体和蚜虫的抵抗力
DOI：
10.3389/fagro.2021.694333
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发表时间：
2021-08-17
影响因子：
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通讯作者：
Ding, Xinhua
中科院分区：
文献类型：
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作者：
Ding, Xiangyu;Zhang, Haimiao;Ding, Xinhua
植物病原体和害虫是限制植物生长的两个主要因素。据报道，类黄酮调节基因 AtMYB12(AT2G47460) 的表达可增加烟草和番茄的内源类黄酮含量。以往的研究仅集中于单一应激条件下v-myb禽类成髓细胞瘤病毒癌基因同源物（MYB）转录因子的调控机制。研究表明，AtMYB12 参与调节烟草对多种生物胁迫（如植物病原体和蚜虫）的抗性。我们报道，当几种防御相关标记（如 NbPR1a、NbNOA1 和 NbrbohB）的上调表达被激活时，携带 AtMYB12 的转基因烟草对青枯菌具有更强的抗性，这表明植物的启动防御可能有助于细菌抗病性。表达AtMYB12的转基因烟草抗性的提高是通过促进ROS、H2O2和NO的产生来实现的。表达AtMYB12的转基因烟草还对真菌病原体具有抗性，例如Colletotrichum nicotianae Averna和Alternaria alterna。黄酮醇成分的富集，例如芦丁，可直接抑制烟草烟草和交替烟草的生长，也可能有助于 AtMYB12 过表达介导的防御。同时，结果还证实，表达AtMYB12的转基因烟草增强了植物对蚜虫侵染（Aphidoidea）害虫的抵抗力。这些结果表明AtMYB12基因是病虫害防治的良好候选基因，其抗性成本有限且黄酮醇富集，并且AtMYB12在抗病烟草作物育种方面具有潜力。
Phytopathogens and pests are two major factors that limit the growth of plants. The expression of a flavonoid regulator gene, AtMYB12(AT2G47460), has been reported to increase the endogenous flavonoid content of tobacco and tomato. Previous research has only focused on the regulation mechanism of v-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors under single stress conditions. Here, research showed that AtMYB12 was involved in regulating the resistance of tobacco to multiple biological stresses such as phytopathogens and aphid. We reported that transgenic tobacco carrying AtMYB12 was more resistant to Ralstonia solanacearum when the up-regulated expression of several defense-related markers, such as NbPR1a, NbNOA1, and NbrbohB, was activated, suggesting that the priming defense of a plant may contribute to bacterial disease resistance. The improvement of the resistance of AtMYB12-expressing transgenic tobacco is achieved by promoting the production of ROS, H2O2, and NO. AtMYB12-expressing transgenic tobacco also has resistance to fungal pathogens, such as Colletotrichum nicotianae Averna and Alternaria alternate. The enrichment of flavonols components, such as rutin, which directly inhibit the growth of C. nicotianae and A. alternate, may also contribute to the defense mediated by AtMYB12 over-expression. At the same time, the results also confirm that AtMYB12-expressing transgenic tobacco enhanced plant resistance to aphid-infested (Aphidoidea) pests. These results suggest that the AtMYB12 gene is a good candidate for pest and disease control, with limited resistance costs and enrichment in flavonols, and that AtMYB12 has a potential in the breeding of disease-resistant tobacco crops.