Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester.
Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester.
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发表时间：
1992-09
影响因子：
2.6
通讯作者：
Mu-Ya Liu;Ming-Chi Wu
中科院分区：
文献类型：
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作者：
Mu-Ya Liu;Ming-Chi Wu
人组织细胞性淋巴瘤细胞系U937可被浓度为8-16 ng/ml的佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）诱导分化。诱导细胞表现出生长抑制，单核-巨噬细胞样细胞的形态变化，以及氮蓝四唑（NBT）和非特异性酯酶活性的增加。巨噬细胞的表面标志物MO 1和MO 2的表达也升高。U937细胞的诱导分化伴随着培养基中集落刺激活性的出现，如在小鼠骨髓细胞上测定的。条件培养基的凝胶过滤色谱显示集落刺激因子（CSF）活性的峰，其表观分子量约为100 kd。集落的形态学分析揭示了主要的单核细胞-巨噬细胞群体。这种CSF活性可以被抗人集落刺激因子1（CSF-1）抗体中和，Western印迹分析显示CSF-1条带。所有这些结果都有力地表明诱导的U937细胞产生的CSF是CSF-1。北方印迹分析表明，PMA诱导3 h后CSF-1基因开始表达，18 h后表达下降。原癌基因c-fos和c-jun在PMA孵育半小时后表达。
The monoblast-like human histiocytic lymphoma cell line U937 can be induced by phorbol 12-myristate 13-acetate (PMA) at concentrations of 8-16 ng/ml to undergo differentiation. The induced cells show growth inhibition, morphological changes to monocyte-macrophage-like cells, and increases in nitroblue tetrazolium (NBT) and nonspecific esterase activities. The expression of surface markers MO1 and MO2 for macrophages is also elevated. The induced differentiation of U937 cells is accompanied by the appearance of colony-stimulating activity in the culture medium as assayed on mouse bone marrow cells. Gel filtration chromatography of the conditioned media shows a peak of colony-stimulating factor (CSF) activity with an apparent molecular weight of approximately 100 kd. Morphological analysis of the colonies reveals a predominant monocyte-macrophage population. This CSF activity can be neutralized by anti-human colony-stimulating factor 1 (CSF-1) antibody, and Western blot analysis shows a CSF-1 band. All these results strongly indicate that CSF produced by induced U937 cells is CSF-1. Northern blot analysis of RNA isolated from U937 cells demonstrated that CSF-1 gene expression started after PMA induction for 3 h and declined after 18 h. Protooncogenes c-fos and c-jun were expressed after half an hour of incubation with PMA.