Control of the acetamidase gene of Mycobacterium smegmatis by multiple regulators
Control of the acetamidase gene of Mycobacterium smegmatis by multiple regulators
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DOI：
10.1016/s0378-1097(03)00177-0
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发表时间：
2003-04-11
影响因子：
2.1
通讯作者：
Parish, T
中科院分区：
文献类型：
--
作者：
Roberts, G;Muttucumaru, DGN;Parish, T
耻垢分枝杆菌的乙酰胺酶是一种诱导酶，使生物体能够利用多种酰胺作为唯一碳源。乙酰胺酶结构基因（amiE）位于其他四个基因的下游，其中三个与amiE形成可能的操纵子；第四个（amiC）是不同转录的。我们在其中两个基因中构建了缺失突变体，以确定它们在乙酰胺酶表达中的作用：AmiC 和 AmiD 均被证明是诱导所需的乙酰胺酶表达的正调节因子。进行了调控基因删除的组合，结果表明 AmiC 与先前表征的负调控因子 AmiA 相互作用，而 AmiD 则不然。 (C) 2003 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
The acetamidase of Mycobacterium smegmatis is an inducible enzyme which enables the organism to utilise several amides as sole carbon sources. The acetamidase structural gene (amiE) is located downstream of four other genes, of which three form a probable operon with amiE; the fourth (amiC) is divergently transcribed. We constructed deletion mutants in two of these genes in order to determine their role in acetamidase expression: Both AmiC and AmiD were shown to be positive regulators of acetamidase expression required for induction. Combinations of regulatory gene deletions were made which revealed that AmiC interacts with the previously characterised negative regulator AmiA, whereas AmiD does not. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.