Pol κ: A DNA Polymerase Required for Sister Chromatid Cohesion
Pol κ: A DNA Polymerase Required for Sister Chromatid Cohesion
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DOI：
10.1126/science.289.5480.774
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发表时间：
2000-08
期刊：
影响因子：
56.9
通讯作者：
Zhenghe Wang;I. Castaño;Alejandro De Las Peñas;C. Adams;M. Christman
中科院分区：
文献类型：
--
作者：
Zhenghe Wang;I. Castaño;Alejandro De Las Peñas;C. Adams;M. Christman
Establishment of cohesion between sister chromatids is coupled to replication fork passage through an unknown mechanism. Here we report that TRF4 , an evolutionarily conserved gene necessary for chromosome segregation, encodes a DNA polymerase with β-polymerase–like properties. A double mutant in the redundant homologs, TRF4 and TRF5 , is unable to complete S phase, whereas a trf4 single mutant completes a presumably defective S phase that results in a failure of cohesion between the replicated sister chromatids. This suggests that TRF s are a key link in the coordination between DNA replication and sister chromatid cohesion. Trf4 and Trf5 represent the fourth class of essential nuclear DNA polymerases (designated DNA polymerase kappa) in Saccharomyces cerevisiae and probably in all eukaryotes.
