Induction of S-nitrosoglutathione reductase reduces root cadmium uptake by inhibiting Iron-regulated transporter 1
Induction of S-nitrosoglutathione reductase reduces root cadmium uptake by inhibiting Iron-regulated transporter 1
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S-亚硝基谷胱甘肽还原酶的诱导通过抑制铁调节转运蛋白 1 减少根部镉的吸收
DOI：
10.1007/s11104-019-04014-z
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发表时间：
2019-05-01
期刊：
影响因子：
4.9
通讯作者：
Jin, Chong Wei
中科院分区：
文献类型：
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作者：
Guan, Mei Yan;Zhu, Ya Xin;Jin, Chong Wei
农业土壤镉污染日益严重是一个严重的问题。如果我们希望提高植物从污染土壤中清除Cd的效率，那么确定控制根系吸收Cd的机制是必不可少的。利用s -亚硝基谷胱甘肽还原酶（GSNOR）相关突变体和药理学方法，研究了GSNOR在调节拟南芥根系Cd吸收中的作用和机制。暴露于Cd刺激了根中GSNOR的活性。在野生型植物中，s -亚硝基谷胱甘肽（GSNOR的底物）处理和GSNOR突变体中GSNOR功能的丧失都能改善铁调节转运蛋白1 （IRT1）的表达，增加根对Cd的吸收，从而提高植物中的Cd水平。在GSNOR过表达的转基因植物GSNOROE中观察到相反的模式，表明GSNOR负调控IRT1表达和Cd摄取。然而，无论是由于s -亚硝基谷胱甘肽处理或GSNOR突变而提高Cd吸收，还是由于GSNOR过表达而抑制Cd吸收，都可能被IRT1功能丧失所阻断。我们认为，GSNOR的诱导减少了Cd的吸收，这是因为GSNOR负调控了根系中的IRT1，从而降低了植物体内Cd的积累。
Increasing cadmium (Cd) contamination of agricultural soils is a serious problem. Identification of the mechanisms that control Cd uptake by roots is essential if we wish to improve the efficiency of plants in removing Cd from contaminated soils. We dissected the role and the mechanism of S-nitrosoglutathione reductase (GSNOR) in regulating root Cd uptake in Arabidopsis plants using GSNOR-related mutants and pharmacological methods. Exposure to Cd stimulated the activity of GSNOR in roots. Both treatment with S-nitrosoglutathione (substrate of GSNOR) in wild-type plants and loss of GSNOR function in gsnor mutants improved expression of IRON-REGULATED TRANSPORTER 1 (IRT1) and increased root Cd uptake, thereby elevating the Cd levels in plants. The opposite patterns were observed in the GSNOR over-expression transgenic plant GSNOROE, suggesting a negative regulation of IRT1 expression and Cd uptake by GSNOR. However, both the improvement of Cd uptake owing to S-nitrosoglutathione treatment or GSNOR mutation and the inhibition of Cd uptake due to GSNOR over-expression, could be blocked by loss of function of IRT1 in plants. We concluded that induction of GSNOR reduced Cd uptake because of its negative regulation of IRT1 in roots, which lowered Cd accumulation in plants.