A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast
A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast
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DOI：
10.1099/mic.0.036723-0
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发表时间：
2010-06-01
期刊：
影响因子：
2.8
通讯作者：
Blondin, Bruno
中科院分区：
文献类型：
--
作者：
Nardi, Tiziana;Corich, Viviana;Blondin, Bruno
亚硫酸盐因其抗微生物和抗氧化活性而被广泛用作食品和饮料中的防腐剂，特别是在经常添加SO(2)的酿酒中。因此，对发酵酵母的亚硫酸盐抗性机制进行了广泛的研究。亚硫酸盐解毒是酿酒酵母最有效的抗性机制，涉及由SSU1基因编码的质膜蛋白。在本研究中，我们鉴定了SSU1在葡萄酒菌株71B中不同寻常的表达模式。我们首次提供了亚硫酸盐诱导SSU1的证据。对发酵和不同生长条件下SSU1表达的研究表明，亚硫酸盐是SSU1表达的主要调控因子，解释了SSU1表达的特殊规律。结合对亚硫酸盐的基因表达和生长行为的分析，我们发现71B在亚硫酸盐预适应中表现出独特的行为模式，这可能与SSU1表达的变化有关。对SSU1基因座的基因组组织和该区域的测序结果显示，71B中有三个不同的等位基因，其中两个对应于易位的VIII-XVI型。启动子区域之间缺乏差异表明，这种可诱导的SSU1表达模式是由于调节/信号通路的修改。
Sulphite is widely used as a preservative in foods and beverages for its antimicrobial and antioxidant activities, particularly in winemaking where SO(2) is frequently added. Thus, sulphite resistance mechanisms have been extensively studied in the fermenting yeast Saccharomyces cerevisiae. Sulphite detoxification, involving a plasma membrane protein encoded by the SSU1 gene, is the most efficient resistance mechanism in S. cerevisiae. In this study, we characterized the unusual expression pattern of SSU1 in the wine strain 71B. We provide, for the first time, evidence of SSU1 induction by sulphite. The study of SSU1 expression during fermentation and in different growth conditions showed that sulphite is the main regulator of SSU1 expression, explaining its specific pattern. Combining analyses of gene expression and growth behaviour in response to sulphite, we found that 71B displayed unique behavioural patterns in response to sulphite pre-adaptation that may be explained by changes in SSU1 expression. Examination of the genomic organization of the SSU1 locus and sequencing of the region revealed three different alleles in 71B, two of which corresponded to translocated VIII-XVI forms. The lack of differences between promoter regions suggests that this inducible SSU1 expression pattern is due to modification of regulatory/signalling pathways.