EPSTEIN-BARR VIRUS-INFECTIONS AND DNA HYBRIDIZATION STUDIES IN POSTTRANSPLANTATION LYMPHOMA AND LYMPHOPROLIFERATIVE LESIONS - THE ROLE OF PRIMARY INFECTION
EPSTEIN-BARR VIRUS-INFECTIONS AND DNA HYBRIDIZATION STUDIES IN POSTTRANSPLANTATION LYMPHOMA AND LYMPHOPROLIFERATIVE LESIONS - THE ROLE OF PRIMARY INFECTION
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DOI：
10.1093/infdis/152.5.876
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
ROSENTHAL, JT
中科院分区：
文献类型：
--
作者：
HO, M;MILLER, G;ROSENTHAL, JT
1981-1983年在匹兹堡进行肾、肝、心脏或心肺移植后发生B细胞淋巴瘤或淋巴组织增生性病变的14例患者，有原发型（6例）、再活化型（7例）或慢性型（1例）EB病毒（EBV）活动性感染。在无肿瘤的移植患者中，EBV感染的发生率为30%（39/128）。这些患者中只有3例有原发性感染。因此，肿瘤患者的活动性感染频率明显较高，原发性感染患者发生肿瘤的风险更大。用BamHI K、BamHI W或EcoRI B克隆探针进行DNA-DNA杂交，13个肿瘤中5个含有EBV核抗原（EBNA），11个肿瘤中9个含有EBV基因组。所有EBNA阳性的肿瘤，除了一个，也是阳性杂交。只有一个肿瘤是阴性的EBNA和EBV DNA。这些数据表明，EBV在这些病变的发展中起着病因学作用。
Fourteen patients who developed B cell lymphomas or lymphoproliferative lesions after kidney, liver, heart, or heart-lung transplantation in Pittsburgh during 1981-1983 had active infection with Epstein-Barr virus (EBV) of the primary (six patients), reactivated (seven patients), or chronic (one patient) type. In transplant patients without tumors, the incidence of EBV infection was 30% (39 of 128). Only three of these patients had primary infections. Thus the frequency of active infection was significantly higher in patients with tumors, and patients with primary infections were at greater risk of developing tumors. Five of 13 tumors tested contained EBV nuclear antigen (EBNA) and nine of 11 contained EBV genomes detected by DNA-DNA hybridization with BamHI K, BamHI W, or EcoRI B cloned probes. All EBNA-positive tumors, except one, were also positive by hybridization. Only one tumor was negative for both EBNA and EBV DNA. These data suggest that EBV plays an etiologic role in the development of these lesions.