Identification of salivary proteins in the whiteflyBemisia tabaciby transcriptomic and LC-MS/MS analyses
Identification of salivary proteins in the whiteflyBemisia tabaciby transcriptomic and LC-MS/MS analyses
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通过转录组学和 LC-MS/MS 分析鉴定烟粉虱唾液蛋白
DOI：
10.1111/1744-7917.12856
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发表时间：
2020-08-26
期刊：
影响因子：
4
通讯作者：
Li, Jun-Min
中科院分区：
文献类型：
--
作者：
Huang, Hai-Jian;Ye, Zhuang-Xin;Li, Jun-Min
烟粉虱是世界上许多农作物的臭名昭著的农业害虫。尽管人们认为b。白粉虱分泌唾液进入寄主植物以对抗植物的防御，关于白粉虱唾液蛋白质组的知识有限。在这里，我们对b的基因/蛋白谱进行了表征。用转录组学和液相色谱串联质谱分析烟盘唾液腺和分泌唾液。共鉴定出698个唾液腺富集单基因和171个唾液腺蛋白。比较分析；Tabacisalivary蛋白与不同节肢动物的蛋白在结合、水解和氧化还原相关的蛋白上有许多相似之处，这表明在食草唾液中存在一定程度的保守性。仅在b中鉴定出74种蛋白。tabacisaliva，其中有34只。tabaci-specific。唾液蛋白13种，其中11种为b。当b。在不同的主机上tabacated。本研究结果为今后粉虱唾液效应剂的功能研究提供了良好的资源，并可用于害虫防治。
The whiteflyBemisia tabaciis a notorious agricultural pest of many crops worldwide. Although it is thought thatB. tabacisecretes saliva into the host plant to counter plant defenses, knowledge on the whitefly salivary proteome is limited. Here, we characterized the gene/protein repertoires ofB. tabacisalivary glands and secreted saliva by transcriptomic and liquid chromatography tandem mass spectroscopy analyses. A total of 698 salivary gland-enriched unigenes and 171 salivary proteins were identified. Comparative analysis between theB. tabacisalivary proteins and those of different arthropod species revealed numerous similarities in proteins associated with binding, hydrolysis, and oxidation-reduction, which demonstrates a degree of conservation across herbivorous saliva. There were 74 proteins only identified inB. tabacisaliva, of which 34 wereB. tabaci-specific. In addition, 13 salivary proteins, of which 11 wereB. tabaci-specific, were differentially regulated whenB. tabacifed on different hosts. Our results provide a good resource for future functional studies of whitefly salivary effectors, and might be useful in pest management.