MEASUREMENT OF FREE AMINO-ACID LEVELS IN ULTRAFILTRATES OF BLOOD-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AUTOMATIC PRECOLUMN DERIVATIZATION
MEASUREMENT OF FREE AMINO-ACID LEVELS IN ULTRAFILTRATES OF BLOOD-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AUTOMATIC PRECOLUMN DERIVATIZATION
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DOI：
10.1016/0378-4347(92)80385-4
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发表时间：
1992-09-04
期刊：
影响因子：
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通讯作者：
WORTHEN, HG
中科院分区：
文献类型：
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作者：
LIU, H;WORTHEN, HG
尽管有许多技术可用于氨基酸分析，但脱蛋白仍然是生理液中氨基酸分析的主要问题之一。制备血浆和去除血浆蛋白的方法对最终结果有显着影响。最广泛使用的脱蛋白方法是用 5-磺基水杨酸沉淀，然后离心去除沉淀的蛋白质。由于磺基水杨酸上清液对定量和分离的不利影响，我们尚未成功使用该脱蛋白剂通过自动柱前邻苯二甲醛-3-巯基丙酸和9-芴基甲基氯甲酸酯衍生高效液相色谱法分析血浆氨基酸。本实验中使用超滤作为制备血浆样品的替代方法。对四年内 1500 个样本的血浆氨基酸分析结果令人满意。研究了可能影响超滤结果的一些因素。
Although there are many techniques available for the analysis of amino acids, deproteinization is still one of the major problems in the analysis of amino acids in physiological fluids. The method used to prepare the plasma and to remove the plasma protein has a marked effect on the final results. The most widely used method of deproteinization is precipitation with 5-sulphosalicylic acid followed by centrifugation to remove the precipitated protein. We have not had success in using this deproteinization agent for the analysis of plasma amino acids by a high-performance liquid chromatographic method with automatic pre-column o-phthaldialdehyde-3-mercaptopropionic acid and 9-fluorenylmethyl chloroformate derivatization because of the adverse effect of the sulphosalicylic acid supernatant on the quantitation and separation. Ultrafiltration was used as an alternative method for the preparation of plasma samples in this experiment. The results were satisfactory for the analysis of plasma amino acids in 1500 samples during a period of four years. Some factors that might influence the results of the ultrafiltration were investigated.