TRANSCRIPTIONAL REGULATION IN THE YEAST LIFE-CYCLE
TRANSCRIPTIONAL REGULATION IN THE YEAST LIFE-CYCLE
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DOI：
10.1126/science.3306917
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发表时间：
1987-09-04
期刊：
影响因子：
56.9
通讯作者：
SHORE, D
中科院分区：
文献类型：
--
作者：
NASMYTH, K;SHORE, D
在同宗配合酵母中从单倍体到二倍体的转变涉及在转录水平上调节的确定的事件序列。SWI基因编码的转录因子在母细胞细胞周期的精确阶段激活HO核酸内切酶基因。HO核酸内切酶启动转座事件，通过使相反交配型的基因远离沉默元件而激活它们。然后，被激活的交配型基因调节参与细胞信号传导的基因，如交配型特异性信息素及其受体。由于HO仅在分裂后的姐妹细胞之一（母亲）中被激活，因此产生相反交配类型的相邻细胞，这些相邻细胞通过诱导参与接合的基因来响应彼此分泌的信息素。这导致二倍体的形成，其中许多参与交配和交配型转换的基因由于交配型位点的杂合性而受到抑制。本文总结了什么是已知的这些转录控制，并讨论了在高等真核生物可能的相似之处。
The transition from haploid to diploid in homothallic yeast involves a defined sequence of events which are regulated at the level of transcription. Transcription factors encoded bySWIgenes activate theHOendonuclease gene at a precise stage in the cell cycle of mother cells. TheHOendonuclease initiates a transposition event which activates genes of the opposite mating type by causing them to move away from a silencer element. The activated mating type genes then regulate genes involved in cell signaling such as the mating type-specific pheromones and their receptors. SinceHOis only activated in one of the sister cells after division (the mother), adjacent cells of opposite mating type are generated which respond to each others' secreted pheromones by inducing genes involved in conjugation. This leads to the formation of a diploid in which many of the genes involved in mating and mating-type switching become repressed due to the heterozygosity of the mating-type locus. This article summarizes what is known about these transcriptional controls and discusses possible parallels in higher eukaryotes.