Gene modules and genes associated with postoperative atrial fibrillation: weighted gene co-expression network analysis and circRNA-miRNA-mRNA regulatory network analysis.
Gene modules and genes associated with postoperative atrial fibrillation: weighted gene co-expression network analysis and circRNA-miRNA-mRNA regulatory network analysis.
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与术后房颤相关的基因模块和基因：加权基因共表达网络分析和circRNA-miRNA-mRNA调控网络分析
DOI：
10.21037/jtd-23-1179
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发表时间：
2023-09-28
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
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作者：
背景心房颤动（AF）是心脏手术患者最常见的并发症。然而，术后房颤（POAF）的发病机制是难以捉摸的，与此相关的研究很少。本研究的目的是在生物信息学分析的基础上，确定POAF的关键基因模块和基因，并对POAF的环状RNA（circRNA）-microRNA（miRNA）-信使RNA（mRNA）调控网络进行分析。方法对GEO数据库中的GSE 143924和GSE 97455数据集进行分析。采用加权基因共表达网络分析（WGCNA）技术，对POAF的关键基因模块和相关基因进行分析。根据差异表达分析，构建了circRNA-miRNA-mRNA调控网络。根据基因本体（GO）和京都基因和基因组百科全书（KEGG）途径分析进一步进行功能富集分析。结果WGCNA共鉴定出2个与POAF相关的关键基因模块和44个关键基因。这些关键基因的功能富集分析涉及以下重要的生物学过程（BP）：内体转运，蛋白激酶B信号传导和转录调控。circRNA-miRNA-mRNA调控网络提示KLF 10可能在POAF中起重要作用。此外，还发现了2个新的circRNA（hsa_circRNA_001654和hsa_circRNA_005899）和2个与KLF 10相关的miRNAs（hsa-miR-19 b-3 p和hsa-miR-30 a-5 p）。结论WGCNA为POAF的发生提供了基础性的表达谱。circRNA-miRNA-mRNA网络提供了对POAF的BP和潜在机制的见解。
Background Atrial fibrillation (AF) is the most common complication in patients undergoing cardiac surgery. However, the pathogenesis of postoperative AF (POAF) is elusive, and research related to this topic is sparse. Our study aimed to identify key gene modules and genes and to conduct a circular RNA (circRNA)-microRNA (miRNA)-messenger RNA (mRNA) regulatory network analysis of POAF on the basis of bioinformatic analysis. Methods The GSE143924 and GSE97455 data sets from the Gene Expression Omnibus (GEO) database were analyzed. Weighted gene co-expression network analysis (WGCNA) was used to identify the key gene modules and genes related to POAF. A circRNA-miRNA-mRNA regulatory network was also built according to differential expression analysis. Functional enrichment analysis was further performed according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results WGCNA identified 2 key gene modules and 44 key genes that were significantly related to POAF. Functional enrichment analysis of these key genes implicated the following important biological processes (BPs): endosomal transport, protein kinase B signaling, and transcription regulation. The circRNA-miRNA-mRNA regulatory network suggested that KLF10 may take critical part in POAF. Moreover, 2 novel circRNAs, hsa_circRNA_001654 and hsa_circRNA_005899, and 2 miRNAs, hsa-miR-19b-3p and hsa-miR-30a-5p, which related with KLF10, were involved in the network. Conclusions Our study provides foundational expression profiles following POAF based on WGCNA. The circRNA-miRNA-mRNA network offers insights into the BPs and underlying mechanisms of POAF.
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影响因子：
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作者：
Yu W;Liu F;Lei Q;Wu P;Yang L;Zhang Y
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Zhang Y
影响因子：
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DOI：
10.1093/europace/eur208
发表时间：
2012-02
期刊：
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology
影响因子：
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作者：
Maesen B;Nijs J;Maessen J;Allessie M;Schotten U
通讯作者：
Schotten U