Single swim sessions in C. elegans induce key features of mammalian exercise.
Single swim sessions in C. elegans induce key features of mammalian exercise.
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DOI：
10.1186/s12915-017-0368-4
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发表时间：
2017-04-10
期刊：
影响因子：
5.4
通讯作者：
Driscoll M
中科院分区：
文献类型：
--
作者：
Laranjeiro R;Harinath G;Burke D;Braeckman BP;Driscoll M
运动对新陈代谢和健康有着非常强大的影响，具有抗疾病和抗衰老的效果。运动有益回路的药理学操作可能会改善久坐和老龄化人群的健康。尽管如此，肌肉运动如何发出信号来促进全系统的健康改善仍然知之甚少。长期以来，我们对促进动物健康改善的干预措施感兴趣，我们试图为秀丽隐杆线虫定义运动选项。在这里，我们报告了单次游泳对C。优美的生理学我们用微量热法证明了C。游泳比爬行消耗更多的能量。连续游泳90分钟的动物专门消耗肌肉脂肪供应，并表现出游泳后运动疲劳，肌肉脂肪消耗和疲劳指标在运动停止后1小时内恢复。定量聚合酶链反应（qPCR）转录分析也表明，在游泳过程中脂肪代谢的增加，随后在运动后几个小时的特定碳水化合物代谢转录下调。在90分钟的游泳过程中，肌肉线粒体基质环境变得更加氧化，可视化的本地化线粒体还原-氧化敏感的绿色荧光蛋白报告。qPCR数据支持在游泳期间和游泳后立即发生的氧化应激防御基因的特异性转录变化。与潜在的抗氧化防御诱导相一致，我们发现，一个单一的游泳课程足以提供保护，防止胡桃醌诱导的氧化应激造成4小时后运动。除了表明即使是一次游泳运动也会带来增加鲁棒性的生理变化外，我们的数据还显示，急性游泳引起的变化与人类报告的一些急性运动反应具有共同特征。总的来说，我们的数据验证了一个容易实现的游泳体验。elegans运动，为利用该模型的实验优势奠定了基础，以遗传学或生物学方法鉴定赋予全系统健康益处的运动相关分子和信号通路。本文的在线版本（doi：10.1186/s12915-017-0368-4）包含补充材料，可供授权用户使用。
Exercise exerts remarkably powerful effects on metabolism and health, with anti-disease and anti-aging outcomes. Pharmacological manipulation of exercise benefit circuits might improve the health of the sedentary and the aging populations. Still, how exercised muscle signals to induce system-wide health improvement remains poorly understood. With a long-term interest in interventions that promote animal-wide health improvement, we sought to define exercise options for Caenorhabditis elegans. Here, we report on the impact of single swim sessions on C. elegans physiology. We used microcalorimetry to show that C. elegans swimming has a greater energy cost than crawling. Animals that swam continuously for 90 min specifically consumed muscle fat supplies and exhibited post-swim locomotory fatigue, with both muscle fat depletion and fatigue indicators recovering within 1 hour of exercise cessation. Quantitative polymerase chain reaction (qPCR) transcript analyses also suggested an increase in fat metabolism during the swim, followed by the downregulation of specific carbohydrate metabolism transcripts in the hours post-exercise. During a 90 min swim, muscle mitochondria matrix environments became more oxidized, as visualized by a localized mitochondrial reduction-oxidation-sensitive green fluorescent protein reporter. qPCR data supported specific transcriptional changes in oxidative stress defense genes during and immediately after a swim. Consistent with potential antioxidant defense induction, we found that a single swim session sufficed to confer protection against juglone-induced oxidative stress inflicted 4 hours post-exercise. In addition to showing that even a single swim exercise bout confers physiological changes that increase robustness, our data reveal that acute swimming-induced changes share common features with some acute exercise responses reported in humans. Overall, our data validate an easily implemented swim experience as C. elegans exercise, setting the foundation for exploiting the experimental advantages of this model to genetically or pharmacologically identify the exercise-associated molecules and signaling pathways that confer system-wide health benefits. The online version of this article (doi:10.1186/s12915-017-0368-4) contains supplementary material, which is available to authorized users.