The pericentriolar satellite protein CEP90 is crucial for integrity of the mitotic spindle pole
The pericentriolar satellite protein CEP90 is crucial for integrity of the mitotic spindle pole
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DOI：
10.1242/jcs.078329
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发表时间：
2011-02-01
影响因子：
4
通讯作者：
Rhee, Kunsoo
中科院分区：
文献类型：
--
作者：
Kim, Kyeongmi;Rhee, Kunsoo
中心周围卫星是集中在中心体周围的电子致密颗粒。它们参与了间期细胞中中心体蛋白的募集和微管的组织，但它们的有丝分裂功能在很大程度上是未知的。在这项研究中，我们将CEP90描述为中心周围卫星的一个组成部分。CEP90既存在于中心体中，也存在于细胞质中，但一旦细胞进入有丝分裂，它就会短暂地集中在中心体上。CEP90缺失导致有丝分裂停止，染色体错位。纺锤极断裂是cep90缺失细胞中最典型的表型。锭子附着后，锭子立即破碎，表明锭子微管的机械力对cep90缺失的锭子结构施加了物理应力。基于这些结果，我们提出CEP90在有丝分裂期间对维持纺锤杆的完整性至关重要。
Pericentriolar satellites are electron-dense granules that are concentrated around the centrosome. They are involved in the recruitment of centrosomal proteins and microtubule organization in interphase cells, but their mitotic functions are largely unknown. In this study, we characterize CEP90 as a component of pericentriolar satellites. CEP90 is present both in the centrosome and in the cytoplasm, but is transiently concentrated at the centrosome once cells enter mitosis. Depletion of CEP90 caused mitotic arrest with misaligned chromosomes. Spindle pole fragmentation was the most characteristic phenotype in CEP90-depleted cells. Spindle poles were fragmented as soon as the spindles attached, suggesting that the mechanical forces of spindle microtubules physically stress the structure of CEP90-depleted spindle poles. Based on these results, we propose that CEP90 is crucial for maintaining the integrity of spindle poles during mitosis.