Modulation of T-Bet and GATA-3 expression in experimental autoimmune thyroiditis rats through ginsenoside treatment
Modulation of T-Bet and GATA-3 expression in experimental autoimmune thyroiditis rats through ginsenoside treatment
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DOI：
10.3109/07435800.2015.1066800
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发表时间：
2016-01-02
影响因子：
2.1
通讯作者：
Huang, Qi
中科院分区：
文献类型：
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作者：
Chen, Jie;Feng, Xiaohong;Huang, Qi
桥本甲状腺炎（HT）是最常见的器官特异性自身免疫性疾病之一。越来越多的证据表明，HT 的特征可能是辅助 T 细胞亚群 Th1 和 Th2 不平衡。中医认为HT是一种慢性疲劳性疾病，导致气虚。在中医中，“气”表示人体脏腑功能的力量；因此中医建议重点治疗HT，以益气生化。人参是一种著名的草药，具有多种功效，其主要功能是生气。人参的主要活性成分是人参皂苷，现代药理学表明，人参皂苷具有双相免疫调节作用，可用于治疗免疫性疾病。既往研究表明人参皂苷对HT具有治疗作用，但其作用机制尚不清楚。为了研究人参皂苷是否可以调节Th1/Th2失衡，制备实验性自身免疫性甲状腺炎大鼠，直接目的是通过ELISA检测IFN-和IL-4的调节作用，并通过实时PCR和Western blot检测T-bet和GATA-3的基因和蛋白表达。 EAT 大鼠中 IFN- 水平升高，而 IL-4 水平降低；人参皂甙治疗导致外周血IFN-水平降低，低剂量具有统计学意义。人参皂苷对IL-4产生双相作用，低剂量和中剂量促进分泌，高剂量抑制分泌。人参皂苷显着降低了 T-bet 的蛋白质和 mRNA 水平，而 GATA-3 则显着增加。
Hashimoto's thyroiditis (HT) is one of the most common organ-specific autoimmune diseases. Increasing evidence indicates that HT may be characterized by an imbalance in the helper T cell subsets Th1 and Th2. Traditional Chinese Medicine (TCM) considers HT as a chronic exhaustion disease, leading to deficiency of qi. In TCM, qi indicates the functional power of the organs of the human body; hence TCM recommends focusing the treatment of HT so as to increase qi production. Ginseng is a well-known herbal medicine exhibiting a variety of efficacies, its main function-being to generate qi. Ginseng's principal active component is ginsenoside, and modern pharmacology has shown that ginsenoside demonstrates biphasic immunomodulatory effects that can be utilized for the treatment of immune disorders. Previous work demonstrated that ginsenoside has a therapeutic effect on HT, but its mechanism is unknown. Experimental autoimmune thyroiditis rats were produced in order to investigate whether ginsenoside can modulate Th1/Th2 imbalance, the direct objective being to examine modulation of IFN- and IL-4 by ELISA, and the gene and protein expression of T-bet and GATA-3 by real-time PCR and Western blot. IFN- levels were increased while IL-4 levels decreased in EAT rats; treatment with ginsenoside led to decreased peripheral blood IFN- levels, with low doses statistically significant. Ginsenoside produced a biphasic effect on IL-4, with low and moderate doses promoting and high doses inhibiting secretion. Both protein and mRNA levels of T-bet were markedly reduced, while GATA-3 was significantly increased by ginsenoside.